Gene Expression Data
Assay Details
Assay
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Reference:
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J:194894
Couesnon A, et al., CLIPR-59: a protein essential for neuromuscular junction stability during mouse late embryonic development. Development. 2013 Apr;140(7):1583-93
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6477283
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Gene symbol:
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Prph
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Gene name:
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peripherin
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Results
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Specimen
6Aa:
embryonic day 18.5; Clip3tm1.1Ics/Clip3 +
(more )
Note: Double labeled: red - Prph; green - alpha-Bungarotoxin.
Specimen
6Aa:
(close )
Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Clip3tm1.1Ics/Clip3+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Prph; green - alpha-Bungarotoxin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: diaphragm |
Present |
Regionally restricted |
6Aa
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Expression in nerve terminals. Pre-synaptic terminals apposed to post-synaptic nAChRs in control and mutant diaphragms.
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Specimen
6Ab:
embryonic day 18.5; Clip3tm1.1Ics/ Clip3tm1.1Ics
(more )
Note: Double labeled: red - Prph; green - alpha-Bungarotoxin.
Specimen
6Ab:
(close )
Genetic Background:
|
involves: C57BL/6
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Mutant Allele(s):
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Clip3tm1.1Ics/Clip3tm1.1Ics
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Age:
|
embryonic day 18.5
|
Sex:
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Not Specified
|
Type:
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section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: red - Prph; green - alpha-Bungarotoxin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: diaphragm |
Present |
Regionally restricted |
6Ab
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Expression in nerve terminals. Pre-synaptic terminals apposed to post-synaptic nAChRs in control and mutant diaphragms.
|
Specimen
6Ac:
embryonic day 18.5; Clip3tm1.1Ics/Clip3 +
(more )
Note: Double labeled: red - Prph; green - alpha-Bungarotoxin.
Specimen
6Ac:
(close )
Genetic Background:
|
involves: C57BL/6
|
Mutant Allele(s):
|
Clip3tm1.1Ics/Clip3+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Prph; green - alpha-Bungarotoxin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: diaphragm |
Present |
Regionally restricted |
6Ac
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Expression in nerve terminals at tip of nerve branch.
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Specimen
6Ad:
embryonic day 18.5; Clip3tm1.1Ics/ Clip3tm1.1Ics
(more )
Note: Double labeled: red - Prph; green - alpha-Bungarotoxin.
Specimen
6Ad:
(close )
Genetic Background:
|
involves: C57BL/6
|
Mutant Allele(s):
|
Clip3tm1.1Ics/Clip3tm1.1Ics
|
Age:
|
embryonic day 18.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Prph; green - alpha-Bungarotoxin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: diaphragm |
Weak |
Regionally restricted |
6Ad
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Thinning of nerve more prononced in mutant than in control, with visible retraction bulbs and ininnervated nAChrs.
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