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Gene Expression Data
Assay Details
Assay
Reference: J:286828 Jiang D, et al., Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae. J Comp Neurol. 2020 Apr 1;528(5):729-755
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6474144
Gene symbol: Hmga1
Gene name: high mobility group AT-hook 1
Antibody: Recombinant Anti-HMGA1 antibody [EPR7839] (ab129153)
Detection system: Secondary antibody
Results
Specimen 5b upper: embryonic day 16.0 (more )
Note: Double labeled: green - Hmga1; magenta - Vsx2 (assay MGI:6474162).
Structure Level Pattern Image Note
TS24: retina inner nuclear layer Present 5b upper merge , 5b upper left 651% were Vsx2-positive.
TS24: retina outer nuclear layer Present 5b upper merge , 5b upper left 651% were Vsx2-positive.
Specimen 5b lower: embryonic day 16.0 (more )
Note: Double labeled: green - Hmga1; magenta - Isl1 (assay MGI:6474172).
Structure Level Pattern Image Note
TS24: retina inner nuclear layer Present 5b lower left , 5b lower merge 402% were Isl1-positive.
TS24: retina outer nuclear layer Present 5b lower left , 5b lower merge 402% were Isl1-positive.
Specimen 5e upper: postnatal day 2 (more )
Note: Double labeled: green - Hmga1; magenta - Vsx2 (assay MGI:6474162).
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present 5e upper merge , 5e upper left
TS27: retina inner nuclear layer Present 5e upper merge , 5e upper left
TS27: retina outer nuclear layer Weak 5e upper left , 5e upper merge Only a few cells coexpressed with Vsx2.
Specimen 5e lower: postnatal day 2 (more )
Note: Double labeled: green - Hmga1; magenta - Isl1 (assay MGI:6474172).
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present 5e lower left , 5e lower merge Around 40% were Isl1+.
TS27: retina inner nuclear layer Present 5e lower left , 5e lower merge Around 40% were Isl1+.
TS27: retina outer nuclear layer Weak 5e lower left , 5e lower merge
Specimen 5h left: postnatal week 14 (more )
Note: Double labeled: green - Hmga1; magenta - Rbpms (assay MGI:6474178).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present 5h left Coexpressed with Rbpms in retina ganglion cells (972%).
TS28: retina inner nuclear layer Present 5h left
TS28: retina outer nuclear layer Weak Scattered 5h left Sparse expression in photoreceptors (31%).
Specimen 5h middle: postnatal week 14 (more )
Note: Double labeled: green - Hmga1; magenta - Vsx2 (assay MGI:6474162).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present 5h middle
TS28: retina inner nuclear layer Present 5h middle Coexpressed with Vsx2 in bipolar cells.
TS28: retina outer nuclear layer Weak Scattered 5h middle Sparse expression in photoreceptors (31%).
Specimen 5h right: postnatal week 14 (more )
Note: Double labeled: green - Hmga1; magenta - Calb1 (assay MGI:6474180).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present 5h right Coexpressed with Calb1 in horizontal cells.
TS28: retina inner nuclear layer Present 5h right Coexpressed with Calb1 in horizontal cells.
TS28: retina outer nuclear layer Weak Scattered 5h right Sparse expression in photoreceptors (31%).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory