Antibody:	Anti-fukutin - N1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex mantle layer	Present	Ubiquitous	1A
TS20: cerebral cortex	Present	Regionally restricted	1A	The germinal matrix was ubiquitously immunolabeled.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		1C	Migrating and settling neurons in the cerebral cortex were immunopositive.
TS24: leptomeninges	Present		1C	The leptomeninges and the pial-glial border were immunopositive.
TS24: brain vascular element	Present	Regionally restricted	1C	Occasional microvessels were immunopositive.

Structure	Level	Pattern	Image	Note
TS24: cerebellum external granule cell layer	Present		1E

Structure	Level	Pattern	Image	Note
TS24: brainstem	Present		2A
TS24: cerebellar cortex	Present		2A
TS24: cochlear duct	Present		2A
TS24: facial nucleus	Present		2A
TS24: medulla oblongata part of 4th ventricle choroid plexus	Present		2A
TS24: medulla oblongata	Present	Regionally restricted	2A	Expression was detected in the subpial pontine migratory stream.

Structure	Level	Pattern	Image	Note
TS24: medulla oblongata	Present	Regionally restricted	2C	Expression was detected in the subpial pontine migratory stream.

Structure	Level	Pattern	Image	Note
TS24: facial nucleus	Present		2E

Structure	Level	Pattern	Image	Note
TS24: pons	Present	Regionally restricted		Expression was detected in the facial nucleus, but was absent from the settled neurons in the pontine nucleus.
TS24: dorsal root ganglion	Present
TS24: spinal cord	Present	Regionally restricted		Expression was detected in the anterior horn cells.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3A
TS28: pia mater	Present		3A

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3C

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		3E
TS28: hippocampus	Present		3E

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		3G

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present		3H

Structure	Level	Pattern	Image	Note
TS28: inferior olivary nucleus	Present
TS28: locus coeruleus	Present
TS28: pontine nucleus	Present
TS28: raphe nucleus	Present
TS28: substantia nigra	Present
TS28: brain nucleus	Present	Regionally restricted		Expression was detected in neuronal somata in the motor and sensory cranial nuclei, and some cells in the reticular formation.