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Gene Expression Data
Assay Details
Assay
Reference: J:288446 Christians A, et al., Inflammation-like changes in the urothelium of Lifr-deficient mice and LIFR-haploinsufficient humans with urinary tract anomalies. Hum Mol Genet. 2020 May 8;29(7):1192-1204
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6472971
Gene symbol: Krt5
Gene name: keratin 5
Antibody: Anti-Keratin 5 (PRB-160P)
Detection system: Alexa Fluor 488
Results
Specimen 1A E14.5 +/+: embryonic day 14.5 (more )
Note: Double labeled: green - Krt5; red - Trp63 (assay MGI:6472976).
Structure Level Pattern Image Note
TS22: intermediate cell layer of ureter Absent 1A middle E14.5 +/+ No expression in the mono-layered urothelium.
Specimen 1A E14.5 -/-: embryonic day 14.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Krt5; red - Trp63 (assay MGI:6472976).
Structure Level Pattern Image Note
TS22: intermediate cell layer of ureter Absent 1A middle E14.5 -/- No expression in the mono-layered urothelium, similar as in the wild-type. A narrowed ureteral lumen was seen.
Specimen 1A E16.5 +/+: embryonic day 16.5 (more )
Note: Double labeled: green - Krt5; red - Trp63 (assay MGI:6472976).
Structure Level Pattern Image Note
TS24: basal cell layer of ureter Present 1A middle E16.5 +/+ Expressed in some cells.
TS24: superficial cell layer of ureter Absent 1A middle E16.5 +/+
Specimen 1A E16.5 -/-: embryonic day 16.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Krt5; red - Trp63 (assay MGI:6472976).
Structure Level Pattern Image Note
TS24: basal cell layer of ureter Present 1A middle E16.5 -/- Expressed in some cells, with a possible slight delay in B-cell differentiation compared with the wild-type.
TS24: superficial cell layer of ureter Absent 1A middle E16.5 -/- Similar as in the wild-type. A narrowed ureteral lumen was seen.
Specimen 1A E18.5 +/+: embryonic day 18.5 (more )
Note: Double labeled: green - Krt5; red - Trp63 (assay MGI:6472976).
Structure Level Pattern Image Note
TS26: basal cell layer of ureter Present 1A middle E18.5 +/+
TS26: intermediate cell layer of ureter Absent 1A middle E18.5 +/+
TS26: superficial cell layer of ureter Absent 1A middle E18.5 +/+
Specimen 1A E18.5 -/-: embryonic day 18.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Krt5; red - Trp63 (assay MGI:6472976).
Structure Level Pattern Image Note
TS26: basal cell layer of ureter Present 1A middle E18.5 -/- Similar as in the wild-type. A narrowed ureteral lumen was seen.
TS26: intermediate cell layer of ureter Absent 1A middle E18.5 -/- Similar as in the wild-type. A narrowed ureteral lumen was seen.
TS26: superficial cell layer of ureter Absent 1A middle E18.5 -/- Similar as in the wild-type. A narrowed ureteral lumen was seen.
Specimen 3A Proxiaml +/+: embryonic day 18.5 (more )
Note: Double labeled: green - Krt5; red - Trp63 (assay MGI:6472976).
Structure Level Pattern Image Note
TS26: basal cell layer of ureter Present 3A left upper +/+
TS26: intermediate cell layer of ureter Absent 3A left upper +/+
TS26: superficial cell layer of ureter Absent 3A left upper +/+
Specimen 3A Proxiaml -/-: embryonic day 18.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Krt5; red - Trp63 (assay MGI:6472976).
Structure Level Pattern Image Note
TS26: basal cell layer of ureter Present 3A left upper -/- Significantly increased numbers of positive cells, compared with the wild-type.
TS26: intermediate cell layer of ureter Absent 3A left upper -/-
TS26: superficial cell layer of ureter Absent 3A left upper -/-
Specimen 3A Distal +/+: embryonic day 18.5 (more )
Note: Double labeled: green - Krt5; red - Trp63 (assay MGI:6472976).
Structure Level Pattern Image Note
TS26: basal cell layer of ureter Present 3A right upper +/+
TS26: intermediate cell layer of ureter Absent 3A right upper +/+
TS26: superficial cell layer of ureter Absent 3A right upper +/+
Specimen 3A Distal -/-: embryonic day 18.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Krt5; red - Trp63 (assay MGI:6472976).
Structure Level Pattern Image Note
TS26: basal cell layer of ureter Present 3A right upper -/- Significantly increased numbers of positive cells, compared with the wild-type.
TS26: intermediate cell layer of ureter Absent 3A right upper -/-
TS26: superficial cell layer of ureter Absent 3A right upper -/-
Specimen 3D +/+: embryonic day 18.5 (more )
Note: Double labeled: green - Krt5; red - Mki67 (assay MGI:6472979).
Structure Level Pattern Image Note
TS26: basal cell layer of ureter Present 3D left +/+
TS26: intermediate cell layer of ureter Absent 3D left +/+
TS26: superficial cell layer of ureter Absent 3D left +/+
Specimen 3D -/-: embryonic day 18.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Krt5; red - Mki67 (assay MGI:6472979).
Structure Level Pattern Image Note
TS26: basal cell layer of ureter Present 3D left -/- Cellular proliferation (Mki67+) was simlar as the wild-type.
TS26: intermediate cell layer of ureter Absent 3D left -/-
TS26: superficial cell layer of bladder urothelium Absent 3D left -/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory