Ddx25 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:294176 Snyder E, et al., ADAD1 and ADAD2, testis-specific adenosine deaminase domain-containing proteins, are required for male fertility. Sci Rep. 2020 Jul 14;10(1):11536
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6472899
Gene symbol:	Ddx25
Gene name:	DEAD box helicase 25

Antibody:

Results

Specimen 5A XI WT: postnatal adult (more

)
Note: Late pachytene spermatocytes (Stage XI). Double labeled: red - Ddx25; green - Smg1 (assay MGI:6472902).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5A WT XI DDX25 , 5A WT XI Merge	Expression is in cytoplasmic granules in late pachytene spermatocytes.

Specimen 5A IV WT: postnatal adult (more

)
Note: Early round spermatids (Stage IV). Double labeled: red - Ddx25; green - Smg1 (assay MGI:6472902).

Structure	Level	Pattern	Image	Note
TS28: spermatid	Present		5A WT IV Merge , 5A WT IV DDX25	Expression is in cytoplasmic granules in early round spermatids.

Specimen 5A XI Adad2^em3/em3: postnatal adult; Adad2^em3(IMPC)J/Adad2^em3(IMPC)J (more

)
Note: Late pachytene spermatocytes (Stage XI). Double labeled: red - Ddx25; green - Smg1 (assay MGI:6472902).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	5A Adad2^em3/em3 XI Merge , 5A Adad2^em3/em3 XI DDX25	Expression in this mutant is the same as in wild-type.

Specimen 5A IV Adad2^em3/em3: postnatal adult; Adad2^em3(IMPC)J/Adad2^em3(IMPC)J (more

)
Note: Early round spermatids (Stage IV). Double labeled: red - Ddx25; green - Smg1 (assay MGI:6472902).

Structure	Level	Pattern	Image	Note
TS28: spermatid	Present	Regionally restricted	5A Adad2^em3/em3 IV Merge , 5A Adad2^em3/em3 IV DDX25	Expression in this mutant is the same as in wild-type.

5B DDX25 ©

5B Merge ©

Specimen 5B: postnatal adult; Adad2^em3(IMPC)J/Adad2^em3(IMPC)J (more

)
Note: Double labeled: red - Ddx25; green - Adad2 (assay MGI:6472838).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5B DDX25 , 5B Merge

5C VII DDX25 ©

5C VII Merge ©

Specimen 5C Stage VII: postnatal adult (more

)
Note: Double labeled: red - Ddx25; green - Adad2 (assay MGI:6472838).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	5C VII Merge , 5C VII DDX25	Expression is in granules in stage IX pachytene spermatocytes.

5C IX DDX25 ©

5C IX Merge ©

Specimen 5C Stage IX: postnatal adult (more

)
Note: Double labeled: red - Ddx25; green - Adad2 (assay MGI:6472838).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	5C IX Merge , 5C IX DDX25	Expression is in granules in stage IX pachytene spermatocytes.

5C XI DDX25 ©

5C XI Merge ©

Specimen 5C Stage XI: postnatal adult (more

)
Note: Double labeled: red - Ddx25; green - Adad2 (assay MGI:6472838).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	5C XI Merge , 5C XI DDX25	Expression is in granules in stage IX pachytene spermatocytes.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/30/2024 MGI 6.23