Gene Expression Data
Assay Details
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Assay
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Reference:
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J:143548
Himes AD, et al., Premature differentiation and aberrant movement of pituitary cells lacking both Hes1 and Prop1. Dev Biol. 2009 Jan 1;325(1):151-61
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6471724
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Gene symbol:
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Cdh2
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Gene name:
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cadherin 2
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Antibody:
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anti-N-cadherin
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
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Results
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Specimen
6B:
embryonic day 13.5
(more  )
Specimen
6B:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: remnant of Rathke's pouch |
Present |
Regionally restricted |
6B
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Expression strongly concentrated along lumen lining dorsal part of Rathke's pouch, but with more dispersed pattern of expression in ventral area where anterior lobe is forming.
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Specimen
6C:
embryonic day 13.5
(more )
Note: Double labeled: red - N-cadherin (Cdh2); green - E-cadherin (Cdh1 assay MGI:6471723).
Specimen
6C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - N-cadherin (Cdh2); green - E-cadherin (Cdh1 assay MGI:6471723).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: remnant of Rathke's pouch |
Present |
Regionally restricted |
6C
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Expression strongly concentrated along lumen lining dorsal part of Rathke's pouch.
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Specimen
6E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: remnant of Rathke's pouch |
Present |
Regionally restricted |
6E
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Expression concentrated along lumen of dorsal half of Rathke's pouch in a smaller proportion of luminal cells than in wildtype.
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - N-cadherin (Cdh2); green - E-cadherin (Cdh1 assay MGI:6471723).
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Prop1df/Prop1df
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: remnant of Rathke's pouch |
Strong |
Regionally restricted |
6H
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Strong expression in lining of nearly entire lumen of Rathke's pouch, except for small area where cells are exiting the pouch for anterior lobe.
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Specimen
6I:
embryonic day 13.5; Prop1df/ Prop1df
(more )
Note: Double labeled: red - N-cadherin (Cdh2); green - E-cadherin (Cdh1 assay MGI:6471723).
Specimen
6I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Prop1df/Prop1df
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - N-cadherin (Cdh2); green - E-cadherin (Cdh1 assay MGI:6471723).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: remnant of Rathke's pouch |
Strong |
Regionally restricted |
6I
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Strong expression in lining of nearly entire lumen of Rathke's pouch, except for small area where cells are exiting the pouch for anterior lobe.
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Specimen
6K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
Prop1df/Prop1df
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: remnant of Rathke's pouch |
Strong |
Regionally restricted |
6K
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Strong expression in lining of dorsal half of lumen of Rathke's pouch. No expression along ventral half of Rathke's pouch where anterior lobe is forming.
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Specimen
6L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
Prop1df/Prop1df
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - N-cadherin (Cdh2); green - E-cadherin (Cdh1 assay MGI:6471723).
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Analysis Tools