Isl1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:143548 Himes AD, et al., Premature differentiation and aberrant movement of pituitary cells lacking both Hes1 and Prop1. Dev Biol. 2009 Jan 1;325(1):151-61
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6471722
Gene symbol:	Isl1
Gene name:	ISL1 transcription factor, LIM/homeodomain

Antibody:	anti-ISL1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Cy3

Results

5E ©

Specimen 5E: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis pars anterior	Present		5E	Expression restricted to anterior lobe in ventral part of pituitary.

5F ©

Specimen 5F: embryonic day 12.5; Hes1^tm1Fgu/Hes1^tm1Fgu (more

)

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis pars anterior	Present		5F	Expression restricted to anterior lobe in ventral part of pituitary.

5G ©

Specimen 5G: embryonic day 12.5; Prop1^df/Prop1^df (more

)

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis pars anterior	Present		5G	Expression restricted to anterior lobe in ventral part of pituitary.

5H ©

Specimen 5H: embryonic day 12.5; Hes1^tm1Fgu/Hes1^tm1Fgu, Prop1^df/Prop1^df (more

)

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis pars anterior	Present		5H	Expression restricted to anterior lobe in ventral part of pituitary.

Specimen E14.5 Wildtype not shown: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis pars anterior	Present	Widespread
TS22: remnant of Rathke's pouch	Present	Widespread

Specimen E14.5 Hes1 mutant not shown: embryonic day 14.5; Hes1^tm1Fgu/Hes1^tm1Fgu (more

)

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis pars intermedia	Present	Widespread		Expression not affected by loss of Hes1 or Prop individually or by loss of both at E14.5.
TS22: remnant of Rathke's pouch	Present	Widespread		Expression not affected by loss of Hes1 or Prop individually or by loss of both at E14.5.

Specimen E14.5 Prop1 mutant not shown: embryonic day 14.5; Prop1^df/Prop1^df (more

)

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis pars anterior	Present	Widespread		Expression not affected by loss of Hes1 or Prop individually or by loss of both at E14.5.
TS22: remnant of Rathke's pouch	Present	Widespread		Expression not affected by loss of Hes1 or Prop individually or by loss of both at E14.5.

Specimen E14.5 Double mutant not shown: embryonic day 14.5; Hes1^tm1Fgu/Hes1^tm1Fgu, Prop1^df/Prop1^df (more

)

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis pars anterior	Present	Widespread		Expression not affected by loss of Hes1 or Prop individually or by loss of both at E14.5.
TS22: remnant of Rathke's pouch	Present	Widespread		Expression not affected by loss of Hes1 or Prop individually or by loss of both at E14.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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