Cga Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:143548 Himes AD, et al., Premature differentiation and aberrant movement of pituitary cells lacking both Hes1 and Prop1. Dev Biol. 2009 Jan 1;325(1):151-61
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6471715
Gene symbol:	Cga
Gene name:	glycoprotein hormones, alpha subunit

Antibody:	alphaGSU antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488

Results

7E ©

Specimen 7E: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis pars anterior	Present		7E	Expression exclusively in anterior lobe in ventral pituitary.

7F ©

Specimen 7F: embryonic day 14.5; Hes1^tm1Fgu/Hes1^tm1Fgu (more

)

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis pars anterior	Present		7F	Expression exclusively in anterior lobe in ventral pituitary.

7G ©

Specimen 7G: embryonic day 14.5; Prop1^df/Prop1^df (more

)

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis pars anterior	Present		7G
TS22: remnant of Rathke's pouch	Present	Regionally restricted	7G	Expression in ventral part of Rathke's pouch.

7H ©

Specimen 7H: embryonic day 14.5; Hes1^tm1Fgu/Hes1^tm1Fgu, Prop1^df/Prop1^df (more

)

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis pars anterior	Present		7H
TS22: remnant of Rathke's pouch	Present	Regionally restricted	7H	Expression in ventral part of Rathke's pouch. Ectopic expressing cells in dorsorostral (drRP) and dorsocaudal (dcRP) Rathke's pouch.

7I ©

Specimen 7I: embryonic day 14.5 (more

)
Note: Double labeled: green - Cga (alphaGSU); red - Snai2 (SLUG assay MGI:6471725).

Structure	Level	Pattern	Image	Note
TS22: pituitary gland	Present	Regionally restricted	7I	Overlap with SLUG (Snai2) expression in occasional cell in rostral tip of pituitary gland.

7J ©

Specimen 7J: embryonic day 14.5; Hes1^tm1Fgu/Hes1^tm1Fgu (more

)
Note: Double labeled: green - Cga (alphaGSU); red - Snai2 (SLUG assay MGI:6471725).

Structure	Level	Pattern	Image	Note
TS22: pituitary gland	Present	Regionally restricted	7J	Overlap with SLUG (Snai2) expression in occasional cell in rostral tip of pituitary gland.

7K ©

Specimen 7K: embryonic day 14.5; Prop1^df/Prop1^df (more

)
Note: Double labeled: green - Cga (alphaGSU); red - Snai2 (SLUG assay MGI:6471725).

Structure	Level	Pattern	Image	Note
TS22: pituitary gland	Present	Regionally restricted	7K	Overlap with SLUG (Snai2) expression in occasional cell in rostral tip of pituitary gland.

7L ©

Specimen 7L: embryonic day 14.5; Hes1^tm1Fgu/Hes1^tm1Fgu, Prop1^df/Prop1^df (more

)
Note: Double labeled: green - Cga (alphaGSU); red - Snai2 (SLUG assay MGI:6471725).

Structure	Level	Pattern	Image	Note
TS22: pituitary gland	Present	Regionally restricted	7L	Overlap with SLUG (Snai2) expression in occasional cell in rostral tip of pituitary gland.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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