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Gene Expression Data
Assay Details
Assay
Reference: J:249893 Nguyen HT, et al., Neurofibromatosis type 1 alternative splicing is a key regulator of Ras/ERK signaling and learning behaviors in mice. Hum Mol Genet. 2017 Oct 01;26(19):3797-3807
Assay type: RT-PCR
MGI Accession ID: MGI:6469683
Gene symbol: Nf1
Gene name: neurofibromin 1
Probe: Nf1-pE, Nf1-pF
Assay notes: Radioactive, semi-quantitative RT-PCR. Primers were designed to measure exon 23a skipping.
Results Image: 2
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
+/+ P adult TS28: brain Present (a) Not Specified; total RNA Not Specified Not Specified
Nf123aIN/+ P adult TS28: brain Present (b) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1+Not Specified
Nf123aIN/Nf123aIN P adult TS28: brain Present (c) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1tm1LuoNot Specified
+/+ P adult TS28: heart Present Not Specified; total RNA Not Specified Not Specified
Nf123aIN/+ P adult TS28: heart Present (b) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1+Not Specified
Nf123aIN/Nf123aIN P adult TS28: heart Present (c) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1tm1LuoNot Specified
+/+ P adult TS28: lung Present Not Specified; total RNA Not Specified Not Specified
Nf123aIN/+ P adult TS28: lung Present (b) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1+Not Specified
Nf123aIN/Nf123aIN P adult TS28: lung Present (c) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1tm1LuoNot Specified
+/+ P adult TS28: metanephros Present Not Specified; total RNA Not Specified Not Specified
Nf123aIN/+ P adult TS28: metanephros Present (b) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1+Not Specified
Nf123aIN/Nf123aIN P adult TS28: metanephros Present (c) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1tm1LuoNot Specified
+/+ P adult TS28: liver Present Not Specified; total RNA Not Specified Not Specified
Nf123aIN/+ P adult TS28: liver Present (b) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1+Not Specified
Nf123aIN/Nf123aIN P adult TS28: liver Present (c) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1tm1LuoNot Specified
+/+ P adult TS28: spleen Present Not Specified; total RNA Not Specified Not Specified
Nf123aIN/+ P adult TS28: spleen Present (b) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1+Not Specified
Nf123aIN/Nf123aIN P adult TS28: spleen Present (c) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1tm1LuoNot Specified
+/+ P adult TS28: skeletal muscle Present Not Specified; total RNA Not Specified Not Specified
Nf123aIN/+ P adult TS28: skeletal muscle Present (b) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1+Not Specified
Nf123aIN/Nf123aIN P adult TS28: skeletal muscle Present (c) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1tm1LuoNot Specified
+/+ P adult TS28: testis Present (d) Not Specified; total RNA Not Specified Male
Nf123aIN/+ P adult TS28: testis Present (b) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1+Male
Nf123aIN/Nf123aIN P adult TS28: testis Present (c) Not Specified; total RNA B6.129-Nf1tm1Luo Nf1tm1Luo/Nf1tm1LuoMale
Notes:
(a) Exon 23a is almost exclusively skipped in WT brain.
(b) Heterozygote mice have an exon 23a inclusion level that stands between that of wild type and mutant.
(c) There is a nearly 100% exon 23a inclusion in this mutant.
(d) Exon 23a is almost exclusively skipped in WT testis.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory