Nf1^tm1Luo
Targeted Allele Detail

Summary

• Symbol: Nf1^tm1Luo
• Name: neurofibromin 1; targeted mutation 1, Guangbin Luo
• MGI ID: MGI:6469615
• Synonyms: Nf1^23aIN, Nf1^23aINneo
• Gene: Nf1 Location: Chr11:79230519-79472438 bp, + strand Genetic Position: Chr11, 46.74 cM, cytoband B4-5
• Alliance: Nf1^tm1Luo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249893
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The 5' splice site (5' SS) and polypyrimidine tract associated with exon 23a were mutated to promote inclusion in the final transcript. A neomycin cassette was inserted into the intron downstream of exon 23a and a second loxP site upstream. (J:249893)

Expression

In Mice Carrying this Mutation:

34 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nf1 Mutation: 157 strains or lines available

References

• Original: J:249893 Nguyen HT, et al., Neurofibromatosis type 1 alternative splicing is a key regulator of Ras/ERK signaling and learning behaviors in mice. Hum Mol Genet. 2017 Oct 01;26(19):3797-3807
• All: 2 reference(s)