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Gene Expression Data
Assay Details
Assay
Reference: J:38635 Lerchner W, et al., Paternal repression of the imprinted mouse Igf2r locus occurs during implantation and is stable in all tissues of the post-implantation mouse embryo. Mech Dev. 1997 Jan;61(1-2):141-9
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6467325
Gene symbol: Igf2r
Gene name: insulin-like growth factor 2 receptor
Reporter: lacZ
Direct Detection
Results
Specimen 1B: embryonic day 13.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the paternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS21: choroid plexus Weak 1B
TS21: heart Weak 1B
TS21: small intestine Weak 1B
TS21: tongue Weak 1B

Specimen 1C: embryonic day 13.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 12 hours staining.
Structure Level Pattern Image Note
TS21: heart Strong 1C Strongest expression.
TS21: small intestine Strong 1C Strongest expression.
TS21: tongue Strong 1C Strongest expression.
TS21: bone Present 1C
TS21: choroid plexus Present 1C
TS21: liver Present 1C
TS21: lung Present 1C
TS21: muscle tissue Present 1C
TS21: skin Present 1C

Specimen 1E: embryonic day 13.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the paternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS21: choroid plexus Weak Regionally restricted 1E Very weak expression in the inner vessels. Staining in the outer layer of the choroid plexus represents endogenous beta-galactosidase activity.

Specimen 1F: embryonic day 13.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 12 hours staining.
Structure Level Pattern Image Note
TS21: choroid plexus Present Regionally restricted 1F Expressed in the inner vessels.

Specimen 1G: embryonic day 17.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 12 hours staining.
Structure Level Pattern Image Note
TS25: skin Present 1G

Specimen 1H: embryonic day 17.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 12 hours staining.
Structure Level Pattern Image Note
TS25: rib Present Regionally restricted 1H Expressed in ossifying tissue.

Specimen 1I: embryonic day 13.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 12 hours staining.
Structure Level Pattern Image Note
TS21: liver Present 1I Expressed in a sub-population of evenly distributed cells.

Specimen 1J: embryonic day 13.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 12 hours staining.
Structure Level Pattern Image Note
TS21: lung mesenchyme Present 1J
TS21: lobar bronchus epithelium Absent 1J

Specimen not shown: embryonic day 17.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 12 hours staining.
Structure Level Pattern Image Note
TS25: heart Present Strongest expression.
TS25: small intestine Present Strongest expression.
TS25: tongue Present Strongest expression.
TS25: bone Present
TS25: choroid plexus Present
TS25: lung mesenchyme Present
TS25: muscle tissue Present
TS25: lobar bronchus epithelium Absent
TS25: liver Present Expressed in a sub-population of evenly distributed cells.

Specimen 2A left: embryonic day 2.0; Igf2rtm1Wag/Igf2r+ (more )
Note: Four-cell embryo. The LacZ allele was from the paternal mutant, after 6 hours staining.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Strong 2A left Expressed in every cell, with different intensities.

Specimen 2A middle: embryonic day 2.5; Igf2rtm1Wag/Igf2r+ (more )
Note: Eight-cell embryo. The LacZ allele was from the paternal mutant, after 6 hours staining.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Strong 2A middle Expressed in every cell, with different intensities.

Specimen 2A right: embryonic day 3.0; Igf2rtm1Wag/Igf2r+ (more )
Note: Early compaction stage. The LacZ allele was from the paternal mutant, after 6 hours staining.
Structure Level Pattern Image Note
TS4: compacted morula Strong 2A right Expressed in every cell, with different intensities.

Specimen not shown: embryonic day 3.5; Igf2rtm1Wag/Igf2r+ (more )
Note: Blastocyst stage. The LacZ allele was from the paternal mutant, after 6 hours staining.
Structure Level Pattern Image Note
TS4: inner cell mass Strong Expressed in every cell, with different intensities.
TS4: trophectoderm Strong Expressed in every cell, with different intensities.

Specimen 2B left: embryonic day 3.5; Igf2rtm1Wag/Igf2r+ (more )
Note: Blastocyst stage. The LacZ allele was from the maternal mutant, after 24 hours staining.
Structure Level Pattern Image Note
TS4: blastocyst Strong 2B left

Specimen 2B middle: embryonic day 3.5; Igf2rtm1Wag/Igf2r+ (more )
Note: Blastocyst stage. The LacZ allele was from the paternal mutant, after 24 hours staining.
Structure Level Pattern Image Note
TS4: blastocyst Strong 2B middle

Specimen 3 E9.5 maternal: embryonic day 9.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 6 hours staining.
Structure Level Pattern Image Note
TS15: allantois Strong 3_E9.5 left
TS15: dorsal aorta Strong 3_E9.5 left
TS15: heart Strong 3_E9.5 left
TS15: limb Strong 3_E9.5 left
TS15: somite Strong 3_E9.5 left
TS15: future midbrain Present Regionally restricted 3_E9.5 left Expressed in a population of cells in the surface.
TS15: telencephalon Present Regionally restricted 3_E9.5 left Expressed in a population of cells in the surface.
TS15: embryo mesoderm Present Regionally restricted 3_E9.5 left Expressed in the lateral mesoderm at the posterior part of the embryo.

Specimen 3 E9.5 paternal left: embryonic day 9.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the paternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS15: heart Weak 3_E9.5 middle
TS15: future midbrain Weak Regionally restricted 3_E9.5 middle Expressed in a population of cells in the surface.
TS15: telencephalon Weak Regionally restricted 3_E9.5 middle Expressed in a population of cells in the surface.

Specimen 3 E9.5 paternal right: embryonic day 9.5; Igf2rtm1Wag/Igf2r+ (more )
Note: Tetralin cleared view. The LacZ allele was from the paternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS15: common cardinal vein Weak 3_E9.5 right
TS15: dorsal aorta Weak 3_E9.5 right
TS15: heart Weak 3_E9.5 right
TS15: future midbrain Weak Regionally restricted 3_E9.5 right Expressed in a population of cells in the surface.
TS15: telencephalon Weak Regionally restricted 3_E9.5 right Expressed in a population of cells in the surface.
TS15: vascular system Weak Regionally restricted 3_E9.5 right All the main vessels.

Specimen 3 E8.5 maternal left: embryonic day 8.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 24 hours staining.
Structure Level Pattern Image Note
TS13: allantois Present 3_E8.5-1
TS13: heart Present 3_E8.5-1

Specimen 3 E8.5 maternal right: embryonic day 8.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 24 hours staining.
Structure Level Pattern Image Note
TS13: allantois Present 3_E8.5-2
TS13: dorsal aorta Present 3_E8.5-2
TS13: heart Present 3_E8.5-2

Specimen 3 E8.5 paternal left: embryonic day 8.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the paternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS13: allantois Weak 3_E8.5-3
TS13: heart Weak 3_E8.5-3

Specimen 3 E8.5 paternal right: embryonic day 8.5; Igf2rtm1Wag/Igf2r+ (more )
Note: Tetralin cleared view. The LacZ allele was from the paternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS13: heart Weak 3_E8.5-4
TS13: vascular system Weak 3_E8.5-4

Specimen 3 E7.5 maternal left: embryonic day 7.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS11: allantois Present 3_E7.5-1
TS11: amnion mesoderm Present 3_E7.5-1
TS11: lateral plate mesoderm Present Regionally restricted 3_E7.5-1 Expressed in the anterior mesoderm.

Specimen 3 E7.5 maternal right: embryonic day 7.5; Igf2rtm1Wag/Igf2r+ (more )
Note: Tetralin cleared view. The LacZ allele was from the maternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS11: allantois Present 3_E7.5-2
TS11: amnion mesoderm Present 3_E7.5-2
TS11: lateral plate mesoderm Present Regionally restricted 3_E7.5-2 Expressed in the anterior mesoderm.

Specimen 3 E7.5 paternal left: embryonic day 7.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the paternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS11: lateral plate mesoderm Weak Regionally restricted 3_E7.5-3 Expressed in the anterior mesoderm.

Specimen 3 E7.5 paternal right: embryonic day 7.5; Igf2rtm1Wag/Igf2r+ (more )
Note: Tetralin cleared view. The LacZ allele was from the paternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS11: lateral plate mesoderm Weak Regionally restricted 3_E7.5-4 Expressed in the anterior mesoderm.

Specimen 3 E6.5 maternal left: embryonic day 6.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the maternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present Regionally restricted 3_E6.5-1 Expressed in the anterior and lateral sides.
TS9: ectoplacental cone Weak 3_E6.5-1

Specimen 3 E6.5 maternal right: embryonic day 6.5; Igf2rtm1Wag/Igf2r+ (more )
Note: Tetralin cleared view. The LacZ allele was from the maternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present Regionally restricted 3_E6.5-2 Expressed in the anterior and lateral sides.
TS9: ectoplacental cone Weak Regionally restricted 3_E6.5-2
TS9: embryo endoderm Weak Regionally restricted 3_E6.5-2
TS9: extraembryonic endoderm Weak 3_E6.5-2

Specimen 3 E6.5 paternal left: embryonic day 6.5; Igf2rtm1Wag/Igf2r+ (more )
Note: The LacZ allele was from the paternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS9: embryo Absent 3_E6.5-3
TS9: extraembryonic component Absent 3_E6.5-3

Specimen 3 E6.5 paternal right: embryonic day 6.5; Igf2rtm1Wag/Igf2r+ (more )
Note: Tetralin cleared view. The LacZ allele was from the paternal mutant, after 36 hours staining.
Structure Level Pattern Image Note
TS9: embryo Absent 3_E6.5-4
TS9: extraembryonic component Absent 3_E6.5-4


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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory