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Gene Expression Data
Assay Details
Assay
Reference: J:155058 Zhang H, et al., Multiple, temporal-specific roles for HNF6 in pancreatic endocrine and ductal differentiation. Mech Dev. 2009 Dec;126(11-12):958-73
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6458834
Gene symbol: Onecut1
Gene name: one cut domain, family member 1
Antibody: anti-HNF6
Results
Specimen 1Ba: embryonic day 11.5; Onecut1tm1.1Mga/Onecut1tm1.1Mga (more )
Note: Double labeled: green - HNF6 (Onecut1); red - Pdx1 (assay MGI:6458836).
Structure Level Pattern Image Note
TS19: pancreas primordium epithelium Present Widespread 1Ba Expression in nuclei of pancreatic epithelium. Expression in all Pdx1-expressing cells.
Specimen 1Bb: embryonic day 11.5; Onecut1tm1.1Mga/Onecut1tm1.1Mga, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Double labeled: green - HNF6 (Onecut1); red - Pdx1 (assay MGI:6458836).
Structure Level Pattern Image Note
TS19: pancreas primordium epithelium Trace Regionally restricted 1Bb Expression in only a few cells. No expression majority of Pdx1-expressing cells.
Specimen 1Bc: embryonic day 13.5; Onecut1tm1.1Mga/Onecut1tm1.1Mga (more )
Note: Double labeled: red - HNF6 (Onecut1); green - Glu (Gcg assay MGI:6458837).
Structure Level Pattern Image Note
TS21: pancreas epithelium Present Widespread 1Bc Expression throughout branching pancreatic epithelium.
Specimen HNF6flox/+:Cre not shown: embryonic day 13.5; Onecut1tm1.1Mga/Onecut1+, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: pancreas epithelium Present Authors report no alteration in expression.
Specimen 1Bd: embryonic day 13.5; Onecut1tm1.1Mga/Onecut1tm1.1Mga, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Double labeled: red - HNF6 (Onecut1); green - Glu (Gcg assay MGI:6458837).
Structure Level Pattern Image Note
TS21: pancreas epithelium Absent 1Bd Expression no longer detectable.
Specimen E13.5 CON not shown: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: pancreas Strong Regionally restricted High levels of expression in large percentage (69%) of Ngn3 (Neurog3)-expressing cells. No expression in glucagon- or insulin-expressing cells at E13.5.
Specimen 2A/A*: embryonic day 15.5 (more )
Note: Double labeled: green - HNF6 (Onecut1); red - Ngn3 (Neurog3; assay MGI:6458838).
Structure Level Pattern Image Note
TS23: pancreas Strong Regionally restricted 2A* , 2A High levels of expression in large percentage (57%) of Ngn3 (Neurog3)-expressing cells. Expression colocalizes with Ngn3 (Neurog3) within endocrine cords.
Specimen 2B: embryonic day 15.5 (more )
Note: Double labeled: green - HNF6 (Onecut1); red - gluc (Gcg assay MGI:6458837).
Structure Level Pattern Image Note
TS23: endocrine pancreas Absent 2B No expression in glucagon-expressing cells.
Specimen 2C: embryonic day 15.5 (more )
Note: Double labeled: green - HNF6 (Onecut1); red - Ins1 (assay MGI:6458840) and Ins2 (assay MGI:6458841).
Structure Level Pattern Image Note
TS23: endocrine pancreas Absent 2C No expression in insulin-expressing cells.
Specimen 2D: embryonic day 15.5 (more )
Note: Double labeled: green - HNF6 (Onecut1); red - Cre reporter.
Structure Level Pattern Image Note
TS23: endocrine pancreas Absent 2D No expression in Amy-expressing cells.
TS23: pancreatic acinus Weak Regionally restricted 2D Lower levels of nuclear expression at distal ductal regions and in acinar cells.
TS23: pancreatic duct Weak Regionally restricted 2D Lower levels of nuclear expression at distal ductal regions and in acinar cells.
Specimen 2E: postnatal day 1 (more )
Note: Double labeled: green - HNF6 (Onecut1); red - gluc (Gcg assay MGI:6458837).
Structure Level Pattern Image Note
TS27: endocrine pancreas Absent 2E No expression in glucagon-expressing cells.
TS27: pancreatic duct Strong Regionally restricted 2E High levels of expression in ductal epithelium.
Specimen 2F: postnatal day 1 (more )
Note: Double labeled: green - HNF6 (Onecut1); red - Ins1 (assay MGI:6458840) and Ins2 (assay MGI:6458841).
Structure Level Pattern Image Note
TS27: endocrine pancreas Absent 2F No expression in insulin-expressing cells.
TS27: pancreatic duct Strong Regionally restricted 2F High levels of expression in ductal epithelium.
Specimen 4E: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Neurog3-cre)C1Able/0 (more )
Note: Conditional mutant. Double labeled: green - HNF6 (Onecut1); red - Cre reporter.
Structure Level Pattern Image Note
TS21: pancreas Present Regionally restricted 4E Co-expression with Bgal in ~79% of Bgal-expressing cells.
Specimen 4F: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Onecut1tm1.1Mga/Onecut1tm1.1Mga, Tg(Neurog3-cre)C1Able/0 (more )
Note: Conditional mutant. Double labeled: green - HNF6 (Onecut1); red - Cre reporter.
Structure Level Pattern Image Note
TS21: pancreas Trace Regionally restricted 4F Co-expression with Bgal in only ~27.5% of Bgal-expressing cells. Majority of Bgal-positive cells have lost HNF6 (Onecut) expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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