Antibody:	Anti-Lyve1
Detection system:	Secondary antibody coupled to Alexa Fluor 350

Structure	Level	Pattern	Image	Note
TS22: jugular lymph sac	Present		4B , 4C , 4E	Cells were found in the developing lymph sacs that were positive for all three markers.

Structure	Level	Pattern	Image	Note
TS22: lymphatic vessel	Present		5A	In developing lymph vessels, a small number of cells were identified that were positive for all three markers. In very rare cases, various marker combinations were observed in adjacent cells of lymph vessels. e.g. 3 adjacent cells: one positive for Adgre1 only, one positive for Adgre1 and Lyve1, and a third positive for all three markers.

Structure	Level	Pattern	Image	Note
TS22: lymphatic vessel	Present		5B	In developing lymph vessels, a small number of cells were identified that were positive for all three markers.