Gene Expression Data
Assay Details
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Assay
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Reference:
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J:136688
Buttler K, et al., Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristics. BMC Dev Biol. 2008;8:43
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6454344
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Gene symbol:
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Lyve1
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Gene name:
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lymphatic vessel endothelial hyaluronan receptor 1
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Antibody:
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Anti-Lyve1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
1E:
embryonic day 13.5
(more  )
Note: Double labeled: red - Lyve1; green - LA102, an epitope on lymphatic endothelial cells.
Specimen
1E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - LA102, an epitope on lymphatic endothelial cells.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: jugular lymph sac |
Present |
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1E
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Lymphatic endothelial cells in the jugular lymph sacs were double positive for Lyve1 and LA102.
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Specimen
1F:
embryonic day 13.5
(more )
Note: Double labeled: red - Lyve1; green - LA102, an epitope on lymphatic endothelial cells.
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - LA102, an epitope on lymphatic endothelial cells.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic vessel |
Present |
Regionally restricted |
1F
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Superficial lymphatic vessels in the dermis were double positive for Lyve1 and LA102.
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Specimen
2D:
embryonic day 12.5
(more )
Note: Double labeled: red - Lyve1; green - LA102, an epitope on lymphatic endothelial cells.
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - LA102, an epitope on lymphatic endothelial cells.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: embryo mesenchyme |
Present |
Regionally restricted |
2D
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Single cells in the mesenchyme surrounding the neural tube co-expressed Lyve1 and LA102.
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Specimen
2F:
embryonic day 13.5
(more )
Note: Double labeled: red - Lyve1; green - Itgam (assay MGI:6454348).
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - Itgam (assay MGI:6454348).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: jugular lymph sac |
Present |
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2F
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Immunopositive lymphatic endothelial cells of the jugular lymph sacs did not co-express Itgam.
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| TS22: embryo mesenchyme |
Present |
Regionally restricted |
2F
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A few Lyve1+/Itgam+ cells were located immediately adjacent to the lymph sacs.
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Specimen
2G:
embryonic day 13.5
(more )
Note: Double labeled: red - Lyve1; green - Itgam (assay MGI:6454348).
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - Itgam (assay MGI:6454348).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic vessel |
Present |
Regionally restricted |
2G
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A few Lyve1+/Itgam+ were detected in the superficial dermal lymph vessels.
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Specimen
3:
embryonic day 13.5
(more )
Note: Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6454672).
Specimen
3:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6454672).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: dermatome |
Present |
Scattered |
3
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A large number of scattered cells co-expressed Lyve1 and Adgre1, a macrophage marker. Some cells expressed only Lyve1 or Adgre1.
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| TS21: mediastinum |
Present |
Scattered |
3
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A large number of scattered cells co-expressed Lyve1 and Adgre1, a macrophage marker. Some cells expressed only Lyve1 or Adgre1.
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| TS21: meninges |
Present |
Scattered |
3
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A large number of scattered cells co-expressed Lyve1 and Adgre1, a macrophage marker. Some cells expressed only Lyve1 or Adgre1.
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Specimen
6A:
embryonic day 11.5
(more )
Note: Double labeled: red - Lyve1; green - Mki67 (assay MGI:6454685).
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - Mki67 (assay MGI:6454685).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: lymph sac |
Present |
Regionally restricted |
6A
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Proliferating lymphatic endothelial cells were noted in the developing jugular lymph sacs.
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| TS19: cardinal vein |
Present |
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6A
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No double positive cells were detected in the cardinal vein.
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Specimen
6B:
embryonic day 11.5
(more )
Note: Double labeled: red - Lyve1; green - Mki67 (assay MGI:6454685).
Specimen
6B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - Mki67 (assay MGI:6454685).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: dermatome |
Present |
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6B
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Two mesenchymal cells were positive for the markers in the dermatome.
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Specimen
6C:
embryonic day 12.5
(more )
Note: Double labeled: red - Lyve1; green - Mki67 (assay MGI:6454685).
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - Mki67 (assay MGI:6454685).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lymph sac |
Present |
Regionally restricted |
6C
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Lymphatic endothelial cells expressing Mki67 and Lyve1 were detected in the jugular lymph sac.
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Specimen
6D:
embryonic day 12.5
(more )
Note: Double labeled: red - Lyve1; green - Mki67 (assay MGI:6454685).
Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - Mki67 (assay MGI:6454685).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lymph sac |
Present |
Regionally restricted |
6D
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A sprout from the jugular lymph sac contained proliferating cells.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: dermatome |
Present |
Scattered |
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Proliferating Lyve1+/Mki67+ cells were detected.
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Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lymph sac |
Present |
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Proliferating Lyve1+/Mki67+ cells were detected in lymph sacs and sprouts from lymph sacs.
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| TS21: dermatome |
Present |
Scattered |
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Proliferating Lyve1+/Mki67+ cells were detected.
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Analysis Tools