Gene Expression Data
Assay Details
Assay
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Reference:
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J:171641
De Maria A, et al., Cadm1 expression and function in the mouse lens. Invest Ophthalmol Vis Sci. 2011;52(5):2293-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6454328
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Gene symbol:
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Cadm1
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Gene name:
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cell adhesion molecule 1
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Antibody:
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anti-Cadm1 (S4945)
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Detection system:
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Secondary antibody
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Assay notes:
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Vibratome sections.
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Results
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Specimen
5A:
postnatal day 3
(more )
Specimen
5A:
(close )
Genetic Background:
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Not Specified
|
Age:
|
postnatal day 3
|
Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Agarose
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Structure |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
Present |
Regionally restricted |
5A
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Expression is in the membranes of nucleated cortical fiber cells and is absent in the central fiber cells.
|
TS27: lens epithelium |
Present |
|
5A
|
|
Specimen
5B:
embryonic day 15.0
(more )
Specimen
5B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lens fiber |
Present |
Regionally restricted |
5B
|
Expression is in the membranes of nucleated cortical fiber cells.
|
Specimen
6A:
postnatal day 3
(more )
Specimen
6A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: lens epithelium |
Present |
Regionally restricted |
6A
|
Expression is in the basolateral membrane domain with little or no expression in the apical membrane.
|
Specimen
6B:
postnatal day 3
(more )
Specimen
6B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
Present |
Regionally restricted |
6B
|
Expression is in young fiber cells near the lens equator in lateral membrane but is absent from the basal membrane.
|
Specimen
6C:
postnatal day 30
(more )
Specimen
6C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 30
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lens fiber |
Present |
|
6C
|
Expression is in the membrane.
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Specimen
6D:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Cadm1tm1.2Brd/Cadm1tm1.2Brd
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Age:
|
postnatal day 30
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
|