Cadm1^tm1.2Brd
Targeted Allele Detail

Summary

• Symbol: Cadm1^tm1.2Brd
• Name: cell adhesion molecule 1; targeted mutation 1.2, Allan Bradley
• MGI ID: MGI:3625158
• Synonyms: Tslc1^Brdm2, Tslc1^m2
• Gene: Cadm1 Location: Chr9:47441471-47769413 bp, + strand Genetic Position: Chr9, 25.83 cM, cytoband B-C
• Alliance: Cadm1^tm1.2Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:108386
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: ES cells targeted with a vector designed to flank exon 9 with loxP sites and insert an FRT-flanked neo were transfected with Cre recombinase. The floxed region including exon 9 and the neo were removed. Exon 9 encodes the transmembrane domain. The skipping of exon 9 resulted in a reading frame shift, which was predicted to result in a nonsense transcript. RT-PCR confirmed that the mutant allele was null. Immunohistochemistry demonstrated the absence of protein in mutant testis. (J:108386)

Expression

In Mice Carrying this Mutation:	4 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Cadm1^tm1.2Brd

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cadm1 Mutation: 39 strains or lines available

References

• Original: J:108386 van der Weyden L, et al., Loss of TSLC1 causes male infertility due to a defect at the spermatid stage of spermatogenesis. Mol Cell Biol. 2006 May;26(9):3595-609
• All: 8 reference(s)