Antibody:	AR antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Results are summarized in Tables 2 and 3.

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Absent		1A

Structure	Level	Pattern	Image	Note
TS21: genital tubercle of male	Present		1B	Four out of 15 males showed positive staining.
TS21: urethral plate epithelium of male	Very strong		1B	Expression is stronger in the more centrally located cells.
TS21: genital tubercle mesenchyme of male	Weak		1B	There is no or weak expression.
TS21: genital tubercle skin of male	Weak		1B	There is no or weak expression.

Structure	Level	Pattern	Image	Note
TS23: genital tubercle of male	Present		1C	Eight out of eight males showed positive staining.
TS23: urethral plate epithelium of male	Very strong		1C	Expression is stronger in the more centrally located cells.
TS23: genital tubercle mesenchyme of male	Moderate		1C	There is moderate to intense expression in the mesenchyme.
TS23: genital tubercle skin of male	Weak		1C	There is no or weak expression.

Structure	Level	Pattern	Image	Note
TS25: genital tubercle of male	Present		1D	Seventeen out of 18 males showed positive staining.
TS25: urethral plate epithelium	Very strong		1D
TS25: genital tubercle mesenchyme of male	Moderate		1D	There is moderate to intense expression.
TS25: genital tubercle skin of male	Weak		1D	There is no or weak expression.

Structure	Level	Pattern	Image	Note
TS27: penis	Present		1E	Twelve out of 12 males showed positive staining.
TS27: urethra epithelium	Very strong		1E
TS27: genital tubercle mesenchyme of male	Moderate		1E	There is moderate to intense expression.
TS27: genital tubercle skin of male	Weak		1E	There is no or weak expression.

Structure	Level	Pattern	Image	Note
TS21: genital tubercle of female	Present		1F	Two out of seven females showed positive staining.
TS21: urethral plate epithelium of female	Very strong		1F	Expression is stronger in the more centrally located cells.
TS21: genital tubercle mesenchyme of female	Weak		1F
TS21: genital tubercle skin of female	Weak		1F

Structure	Level	Pattern	Image	Note
TS23: genital tubercle of female	Present		1G	Eight out of 11 females showed positive staining.
TS23: urethral plate epithelium of female	Very strong		1G	Expression is stronger in the more centrally located cells.
TS23: genital tubercle mesenchyme of female	Moderate		1G	There is moderate to intense expression in the mesenchyme.
TS23: genital tubercle skin of female	Weak		1G	There is no or weak expression.

Structure	Level	Pattern	Image	Note
TS25: genital tubercle of female	Present		1H	Fourteen out of 16 females showed positive staining.
TS25: genital tubercle mesenchyme of female	Moderate		1H	There is moderate to intense expression.
TS25: genital tubercle skin of female	Weak		1H	There is no or weak expression.
TS25: urethral plate epithelium	Weak		1H	There is no or weak expression.

Structure	Level	Pattern	Image	Note
TS27: genital tubercle of female	Present		1I	Eleven out of 11 females showed positive staining.
TS27: genital tubercle mesenchyme of female	Moderate		1I	There is moderate to intense expression.
TS27: genital tubercle skin of female	Weak		1I	There is no or weak expression.
TS27: urethra epithelium	Weak		1I	There is no or weak expression.