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Gene Expression Data
Assay Details
Assay
Reference: J:231259 Krill KT, et al., Dicer deficiency reveals microRNAs predicted to control gene expression in the developing adrenal cortex. Mol Endocrinol. 2013 May;27(5):754-68
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6451189
Gene symbol: Pcna
Gene name: proliferating cell nuclear antigen
Antibody: anti-PCNA
Detection system: Secondary antibody coupled to DyLight 549
Results
Specimen 2A/B 14.5 Cre- WT: embryonic day 14.5; Dicer1tm1Bdh/? (more )
Note: Double labeled: red - Pcna; green - Nr5a1 (assay MGI:6451184). Littermate controls could be flox/flox, or flox/+.
Structure Level Pattern Image Note
TS22: adrenal gland Present 2A upper left , 2B upper left
Specimen 2A/B 14.5 Cre+ KO: embryonic day 14.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Pcna; green - Nr5a1 (assay MGI:6451184).
Structure Level Pattern Image Note
TS22: adrenal gland Present 2A lower left , 2B lower left Same as in the control.
Specimen 2A/B 16.5 Cre- WT: embryonic day 16.5; Dicer1tm1Bdh/? (more )
Note: Double labeled: red - Pcna; green - Nr5a1 (assay MGI:6451184). Littermate controls could be flox/flox, or flox/+.
Structure Level Pattern Image Note
TS24: adrenal gland Present 2A upper middle , 2B upper middle
Specimen 2A/B 16.5 Cre+ KO: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Pcna; green - Nr5a1 (assay MGI:6451184).
Structure Level Pattern Image Note
TS24: adrenal gland Present 2B lower middle , 2A lower middle Same as in the control.
Specimen 2A/B 18.5 Cre- WT: embryonic day 18.5; Dicer1tm1Bdh/? (more )
Note: Double labeled: red - Pcna; green - Nr5a1 (assay MGI:6451184). Littermate controls could be flox/flox, or flox/+.
Structure Level Pattern Image Note
TS26: adrenal gland Present 2B upper right , 2A upper right
Specimen 2A/B 18.5 Cre+ KO: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Pcna; green - Nr5a1 (assay MGI:6451184).
Structure Level Pattern Image Note
TS26: adrenal gland Present Regionally restricted 2A lower right , 2B lower right Overall expression did not differ between control and mutant, but the number of cells coexpressed with Nr5a1 appeared to be somewhat diminished.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory