Tubg1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:284772 DiStasio A, et al., Nubp2 is required for cranial neural crest survival in the mouse. Dev Biol. 2020 Feb 15;458(2):189-199
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6450936
Gene symbol:	Tubg1
Gene name:	tubulin, gamma 1

Antibody:	anti-gamma-tubulin (GTU-88, T6557)
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	The specificity of the antibody used was not detailed; both family members have been annotated.

Results

4A ©

4C ©

Specimen 4A/C: embryonic day 9.5 (more

)
Note: Littermate control; exact genotype not specified. Double labeled: green - Tubg1 and Tubg2; red - Arl13b (assay MGI:6450544).

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	4A , 4C	Expressed in the centrioles.

4E ©

4G ©

Specimen 4E/G: embryonic day 9.5; Nubp2^{tm1c(EUCOMM)Hmgu}/Nubp2^{tm1c(EUCOMM)Hmgu}, H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺, Tg(Wnt1-GAL4)11Rth/0 (more

)
Note: Conditional mutant. Conditional knockout; genotype might be flox/-; Cre. Double labeled: green - Tubg1 and Tubg2; red - Arl13b (assay MGI:6450544).

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	4E , 4G	Expressed in the centrioles. There was no observed difference in the proportion of centrioles per cell in mutants.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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