Antibody:	anti-BHLHB5 (E17; sc-6045)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	8G , 8E	Expression was detected in the type 2 bipolar cells. In addition, a subset of Hcn4-positive type 3a cells was weakly labeled. A population of amacrine cells were also positive.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Weak	Regionally restricted	8H , 8F	A significant reduction of expression was observed in the type 2 bipolar cells. A subset of weakly labeled Hcn4-positive type 3a cells was smaller in size in the mutant. Expression in the amacrine cells was unchanged in the mutant.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Weak	Regionally restricted	S4_merge Irx6LacZ/LacZ;Vsx1+/AltB5 , S4_Bhlhb5 Irx6LacZ/LacZ;Vsx1+/AltB5	Reduced expression was detected in type 2 bipolar cells. Bhlhe22 immunolabeling is present in some of the Irx6:bgal-positive cells that do not co-immunolabel Hcn4.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Weak	Regionally restricted	S4_Bhlhb5 Irx6LacZ/LacZ;Vsx1AltB5/AltB5 , S4_merge Irx6LacZ/LacZ;Vsx1AltB5/AltB5	Reduced expression was detected in type 2 bipolar cells.