Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:189955
Star EN, et al., Regulation of retinal interneuron subtype identity by the Iroquois homeobox gene Irx6. Development. 2012 Dec;139(24):4644-55
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:6450541
|
Gene symbol:
|
Syt2
|
Gene name:
|
synaptotagmin II
|
Antibody:
|
znp-1
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
|
Results
|
Specimen
5C:
postnatal month 2-3; Irx6tm1Chow/Irx6 +
(more )
Specimen
5C:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
|
Mutant Allele(s):
|
Irx6tm1Chow/Irx6+
|
Age:
|
postnatal month 2-3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5D:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
|
Mutant Allele(s):
|
Irx6tm1Chow/Irx6tm1Chow
|
Age:
|
postnatal month 2-3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
S3B:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129S1/SvImj * 129X1/SvJ
|
Mutant Allele(s):
|
Irx6tm1Chow/Irx6tm1Chow
|
Age:
|
postnatal month 2-3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Syt2; green - Irx6 (assay MGI:6450171).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina inner plexiform layer |
Present |
Regionally restricted |
S3C
,
S3B
|
Syt2 immunolabeled axons in the upper and lower zones of the inner plexiform layer corresponding to the OFF and ON projecting region were co-labeled with Irx6:beta-gal.
|
|