Hmgb1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:138375 Cui XS, et al., High mobility group box 1 (HMGB1) is implicated in preimplantation embryo development in the mouse. Mol Reprod Dev. 2008 Aug;75(8):1290-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:6449703
Gene symbol:	Hmgb1
Gene name:	high mobility group box 1

Probe:	Hmgb1-pA, Hmgb1-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Rabbit Globin mRNA was used as an external reference for the analysis of relative mRNA levels. The level of mRNA expression in the blastocyst was arbitrarily set at onefold.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	158 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
1C	E0.5 (a)	TS1: 1-cell stage conceptus	Present	Not Specified; poly-A+ RNA	involves: C57BL/6 * DBA/2		Not Specified
2C	E1.0 (a)	TS2: 2-cell stage conceptus	Present	Not Specified; poly-A+ RNA	involves: C57BL/6 * DBA/2		Not Specified
4C	E2.0 (a)	TS3: 4-cell stage conceptus	Present	Not Specified; poly-A+ RNA	involves: C57BL/6 * DBA/2		Not Specified
MO	E3.0 (a)	TS3: morula-stage conceptus	Present	Not Specified; poly-A+ RNA	involves: C57BL/6 * DBA/2		Not Specified
BL	E3.5 (a)	TS4: blastocyst	Present	Not Specified; poly-A+ RNA	involves: C57BL/6 * DBA/2		Not Specified

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Hmgb1 mRNA levels were high in zygotes, and decreased at the 2-cell stage, but then gradually increased from the 4-cell to blastocyst stage.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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