Antibody:	anti-fimbrin R163.3
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The antibody used likely recognizes all family members; the authors did not detail which member(s) are expressed in the examined tissues; all have been annotated.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present	Regionally restricted	4A/B	Punctate distribution of expression in cytoplasm, with ring of submembranous expression. No expression in germinal vesicle.
TS28: ovary	Weak	Diffuse	4A/B	Weak, diffuse expression in follicle cell surrounding oocytes and adjacent ovarian tissues.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present	Regionally restricted	4C/D	In later-stage oocytes, expression concentrated in cortical cytoplasm.
TS28: ovary	Weak	Diffuse	4C/D	Weak, diffuse expression in follicle cell surrounding oocytes and adjacent ovarian tissues.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		4E	Expression in maternal decidual cells.

Structure	Level	Pattern	Image	Note
TS7: egg cylinder	Present	Widespread	4E	Expression in inner cell mass. Authors report expression in all embryonic and extraembryonic tissues.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present	Diffuse	4F	Diffuse cytoplasmic expression in epithelial lining of foregut.

Structure	Level	Pattern	Image	Note
TS13: yolk sac endoderm	Present	Regionally restricted	4G	Expression redistributed to apical cytoplasm of visceral endoderm.

Structure	Level	Pattern	Image	Note
TS17: yolk sac visceral endoderm	Present	Regionally restricted	4H	Localization of expression to apical surface more prominent by E10.5.

Structure	Level	Pattern	Image	Note
TS17: duodenum	Present	Regionally restricted	7a	Expression more evenly distributed throughout intestinal cells; slightly more concentrated along basal surface of epithelium.

Structure	Level	Pattern	Image	Note
TS20: duodenum epithelium	Present	Regionally restricted	7b	Expression distributed in cytoplasm.

Structure	Level	Pattern	Image	Note
TS23: duodenum epithelium	Present	Regionally restricted	7c	Expression concentrated in apical cytoplasm.
TS23: gut muscularis	Present	Regionally restricted	7c	Expression in smooth muscle surrounding gut.

Structure	Level	Pattern	Image	Note
TS28: duodenum epithelium	Present	Regionally restricted	7d	Expression remains concentrated in apical cytoplasm.