Gene Expression Data
Assay Details
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Assay
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Reference:
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J:283416
Byun H, et al., Rorbeta regulates selective axon-target innervation in the mammalian midbrain. Development. 2019 Jul 22;146(14):dev171926
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6448484
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Gene symbol:
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Rorb
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Gene name:
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RAR-related orphan receptor beta
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Reporter:
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GFP
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Antibody:
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anti-GFP (ab3080)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
S1A E13:
embryonic day 13.0; Rorbtm1.1Df/Rorb +
(more  )
Specimen
S1A E13:
(close  )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
Regionally restricted |
S1A E13
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Expression in a few cells in the superficial layer of superior colliculus.
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Specimen
S1A Rorb:
embryonic day 13.0; Rorbtm1.1Df/Rorb +
(more )
Specimen
S1A Rorb:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
Single cells |
S1A Rorb
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Expression in a few cells in the superficial layer of superior colliculus.
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| TS21: midbrain ventricular layer |
Ambiguous |
Regionally restricted |
S1A Rorb
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Expression barely detectable in ventricular zone/subventricular zone of superior colliculus.
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Specimen
S1B E16:
embryonic day 16.0; Rorbtm1.1Df/Rorb +
(more )
Specimen
S1B E16:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1B P1:
postnatal day 1; Rorbtm1.1Df/Rorb +
(more )
Specimen
S1B P1:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1B P6:
postnatal day 6; Rorbtm1.1Df/Rorb +
(more )
Specimen
S1B P6:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S2C:
postnatal day 1; Rorbtm1.1Df/Rorb +
(more )
Note: Double labeled: green - Rorb reporter; magenta - neurofilament.
Specimen
S2C:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rorb reporter; magenta - neurofilament.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: superior colliculus |
Present |
Regionally restricted |
S2C
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Expression enriched in upper layer of superficial layer.
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Specimen
S2D:
postnatal day 1; Rorbtm1.1Df/ Rorbtm1.1Df
(more )
Note: Double labeled: green - Rorb reporter; magenta - neurofilament.
Specimen
S2D:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorbtm1.1Df
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rorb reporter; magenta - neurofilament.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: superior colliculus |
Present |
Regionally restricted |
S2D
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Expression enriched in upper layer of superficial layer.
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Specimen
S2E:
postnatal day 12; Rorbtm1.1Df/Rorb +
(more )
Note: Double labeled: green - Rorb reporter; magenta - neurofilament.
Specimen
S2E:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rorb reporter; magenta - neurofilament.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: superior colliculus |
Present |
Regionally restricted |
S2E
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Expression in superficial layer of superior colliculus (sSC).
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Specimen
S2 Control inset:
postnatal day 12; Rorbtm1.1Df/Rorb +
(more )
Specimen
S2 Control inset:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S2F:
postnatal day 12; Rorbtm1.1Df/ Rorbtm1.1Df
(more )
Note: Double labeled: green - Rorb reporter; magenta - neurofilament.
Specimen
S2F:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorbtm1.1Df
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rorb reporter; magenta - neurofilament.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: superior colliculus |
Present |
Regionally restricted |
S2F
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Authors report narrowing of superficial layer (sSC).
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Specimen
S2 KO inset:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorbtm1.1Df
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S3D E16 Control:
embryonic day 16.0; Rorbtm1.1Df/Rorb +
(more )
Note: Double labeled: green - Rorb reporter; magenta - Casp3 (assay MGI:6448486).
Specimen
S3D E16 Control:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rorb reporter; magenta - Casp3 (assay MGI:6448486).
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Specimen
S3D E16 cKO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2(cre)Wrst/En1+
Rorbtm3.1Df/Rorbtm3.1Df
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Double labeled: green - Rorb reporter; magenta - Casp3 (assay MGI:6448486).
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Specimen
S3D P1 Control:
postnatal day 1; Rorbtm1.1Df/Rorb +
(more )
Note: Double labeled: green - Rorb reporter; magenta - Casp3 (assay MGI:6448486).
Specimen
S3D P1 Control:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Rorb reporter; magenta - Casp3 (assay MGI:6448486).
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Specimen
S3D P1 cKO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2(cre)Wrst/En1+
Rorbtm3.1Df/Rorbtm3.1Df
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Double labeled: green - Rorb reporter; magenta - Casp3 (assay MGI:6448486).
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Specimen
S3G E16 Control:
embryonic day 16.0; Rorbtm1.1Df/Rorb +
(more )
Specimen
S3G E16 Control:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S3G E16 cKO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2(cre)Wrst/En1+
Rorbtm3.1Df/Rorbtm3.1Df
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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Specimen
S3G P1 Control:
postnatal day 1; Rorbtm1.1Df/Rorb +
(more )
Specimen
S3G P1 Control:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S3G P1 cKO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2(cre)Wrst/En1+
Rorbtm3.1Df/Rorbtm3.1Df
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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Specimen
S3G P5 Control:
postnatal day 5; Rorbtm1.1Df/Rorb +
(more )
Specimen
S3G P5 Control:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S3G P5 cKO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2(cre)Wrst/En1+
Rorbtm3.1Df/Rorbtm3.1Df
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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Specimen
S3G P12 Control:
postnatal day 12; Rorbtm1.1Df/Rorb +
(more )
Specimen
S3G P12 Control:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Rorbtm1.1Df/Rorb+
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S3G P12 cKO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2(cre)Wrst/En1+
Rorbtm3.1Df/Rorbtm3.1Df
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Age:
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postnatal day 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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