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Gene Expression Data
Assay Details
Assay
Reference: J:283416 Byun H, et al., Rorbeta regulates selective axon-target innervation in the mammalian midbrain. Development. 2019 Jul 22;146(14):dev171926
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6448484
Gene symbol: Rorb
Gene name: RAR-related orphan receptor beta
Reporter: GFP
Antibody: anti-GFP (ab3080)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S1A E13: embryonic day 13.0; Rorbtm1.1Df/Rorb+ (more )
Structure Level Pattern Image Note
TS21: midbrain Present Regionally restricted S1A E13 Expression in a few cells in the superficial layer of superior colliculus.
Specimen S1A Rorb: embryonic day 13.0; Rorbtm1.1Df/Rorb+ (more )
Structure Level Pattern Image Note
TS21: midbrain Present Single cells S1A Rorb Expression in a few cells in the superficial layer of superior colliculus.
TS21: midbrain ventricular layer Ambiguous Regionally restricted S1A Rorb Expression barely detectable in ventricular zone/subventricular zone of superior colliculus.
Specimen S1B E16: embryonic day 16.0; Rorbtm1.1Df/Rorb+ (more )
Structure Level Pattern Image Note
TS24: superior colliculus Present Regionally restricted S1B E16 Expression increased in top layer, close to pia.
Specimen S1B P1: postnatal day 1; Rorbtm1.1Df/Rorb+ (more )
Structure Level Pattern Image Note
TS27: superior colliculus Present Regionally restricted S1B P1 Expression remains in top layer.
Specimen S1B P6: postnatal day 6; Rorbtm1.1Df/Rorb+ (more )
Structure Level Pattern Image Note
TS28: superior colliculus Present Regionally restricted S1B P6 Expression remains in top layer.
Specimen S2C: postnatal day 1; Rorbtm1.1Df/Rorb+ (more )
Note: Double labeled: green - Rorb reporter; magenta - neurofilament.
Structure Level Pattern Image Note
TS27: superior colliculus Present Regionally restricted S2C Expression enriched in upper layer of superficial layer.
Specimen S2D: postnatal day 1; Rorbtm1.1Df/Rorbtm1.1Df (more )
Note: Double labeled: green - Rorb reporter; magenta - neurofilament.
Structure Level Pattern Image Note
TS27: superior colliculus Present Regionally restricted S2D Expression enriched in upper layer of superficial layer.
Specimen S2E: postnatal day 12; Rorbtm1.1Df/Rorb+ (more )
Note: Double labeled: green - Rorb reporter; magenta - neurofilament.
Structure Level Pattern Image Note
TS28: superior colliculus Present Regionally restricted S2E Expression in superficial layer of superior colliculus (sSC).
Specimen S2 Control inset: postnatal day 12; Rorbtm1.1Df/Rorb+ (more )
Structure Level Pattern Image Note
TS28: superior colliculus Present Regionally restricted S2_Control inset Expression in neurons in stratum griseum superficiale (SGS) layer.
Specimen S2F: postnatal day 12; Rorbtm1.1Df/Rorbtm1.1Df (more )
Note: Double labeled: green - Rorb reporter; magenta - neurofilament.
Structure Level Pattern Image Note
TS28: superior colliculus Present Regionally restricted S2F Authors report narrowing of superficial layer (sSC).
Specimen S2 KO inset: postnatal day 12; Rorbtm1.1Df/Rorbtm1.1Df (more )
Structure Level Pattern Image Note
TS28: superior colliculus Present Regionally restricted S2_KO inset Decrease in distribution and number of expressing cells.
Specimen S3D E16 Control: embryonic day 16.0; Rorbtm1.1Df/Rorb+ (more )
Note: Double labeled: green - Rorb reporter; magenta - Casp3 (assay MGI:6448486).
Structure Level Pattern Image Note
TS24: superior colliculus Present S3D E16 Control (left)
Specimen S3D E16 cKO: embryonic day 16.0; En1tm2(cre)Wrst/En1+, Rorbtm3.1Df/Rorbtm3.1Df (more )
Note: Conditional mutant. Double labeled: green - Rorb reporter; magenta - Casp3 (assay MGI:6448486).
Structure Level Pattern Image Note
TS24: superior colliculus Present S3D E16 cKO (left) No difference in layer distribution of expressing cells between genotypes.
Specimen S3D P1 Control: postnatal day 1; Rorbtm1.1Df/Rorb+ (more )
Note: Double labeled: green - Rorb reporter; magenta - Casp3 (assay MGI:6448486).
Structure Level Pattern Image Note
TS27: superior colliculus Present S3D P1 Control (left) Number of expressing neurons decreased from embryonic to postnatal stages.
Specimen S3D P1 cKO: postnatal day 1; En1tm2(cre)Wrst/En1+, Rorbtm3.1Df/Rorbtm3.1Df (more )
Note: Conditional mutant. Double labeled: green - Rorb reporter; magenta - Casp3 (assay MGI:6448486).
Structure Level Pattern Image Note
TS27: superior colliculus Present S3D P1 cKO (left) No difference in layer distribution of expressing cells between genotypes.
Specimen S3G E16 Control: embryonic day 16.0; Rorbtm1.1Df/Rorb+ (more )
Structure Level Pattern Image Note
TS24: superior colliculus Present S3G E16 (left)
Specimen S3G E16 cKO: embryonic day 16.0; En1tm2(cre)Wrst/En1+, Rorbtm3.1Df/Rorbtm3.1Df (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: superior colliculus Present S3G E16 (right) No difference in layer distribution of expressing cells between genotypes.
Specimen S3G P1 Control: postnatal day 1; Rorbtm1.1Df/Rorb+ (more )
Structure Level Pattern Image Note
TS27: superior colliculus Present S3G P1 (left)
Specimen S3G P1 cKO: postnatal day 1; En1tm2(cre)Wrst/En1+, Rorbtm3.1Df/Rorbtm3.1Df (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: superior colliculus Present S3G P1 (right) No difference in layer distribution of expressing cells between genotypes.
Specimen S3G P5 Control: postnatal day 5; Rorbtm1.1Df/Rorb+ (more )
Structure Level Pattern Image Note
TS28: superior colliculus Present S3G P5 (left)
Specimen S3G P5 cKO: postnatal day 5; En1tm2(cre)Wrst/En1+, Rorbtm3.1Df/Rorbtm3.1Df (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: superior colliculus Present S3G P5 (right) No difference in layer distribution of expressing cells between genotypes.
Specimen S3G P12 Control: postnatal day 12; Rorbtm1.1Df/Rorb+ (more )
Structure Level Pattern Image Note
TS28: superior colliculus Present S3G P12 (left)
Specimen S3G P12 cKO: postnatal day 12; En1tm2(cre)Wrst/En1+, Rorbtm3.1Df/Rorbtm3.1Df (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: superior colliculus Present S3G P12 (right) No difference in layer distribution of expressing cells between genotypes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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