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Gene Expression Data
Assay Details
Assay
Reference: J:278346 Winkler CC, et al., Loss of Shh signaling in the neocortex reveals heterogeneous cell recovery responses from distinct oligodendrocyte populations. Dev Biol. 2019 Aug 1;452(1):55-65
Assay type: RNA in situ
MGI Accession ID: MGI:6448415
Gene symbol: Smo
Gene name: smoothened, frizzled class receptor
Probe: Mm-Smo (318411)
Probe preparation: Antisense labelled with other - see notes
Visualized with: Fluorescence
Assay notes: RNAscope assay. Visualization was with Opal 570 reagent.
Results
Specimen 2D Example 1: embryonic day 17.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Double labeled: blue - Smo; red - Olig2 (assay MGI:6448412).
Structure Level Pattern Image Note
TS25: neocortex Present 2D Example 1 Smo , 2D Example 1 Merge
Specimen 2D Example 2: embryonic day 17.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Double labeled: blue - Smo; red - Olig2 (assay MGI:6448412).
Structure Level Pattern Image Note
TS25: neocortex Present 2D Example 2 Merge , 2D Example 2 Smo
Specimen 2D Example 3: embryonic day 17.5; Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/Gt(ROSA)26Sor+, Smotm2Amc/Smotm2Amc (more )
Note: Conditional mutant. Double labeled: blue - Smo; red - Olig2 (assay MGI:6448412).
Structure Level Pattern Image Note
TS25: neocortex Present 2D Example 3 Smo , 2D Example 3 Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory