Antibody:	anti-Ad4BP
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS23: testis		Present	Regionally restricted	1A , 1A'	Expression was detected in somatic cells.
TS23: Leydig cell	Leydig cell	Strong	Regionally restricted	1A' , 1A	Strong expression was found on Leydig cells around the testis cords.
TS23: Sertoli cell	Sertoli cell	Strong		1A' , 1A
TS23: rete testis		Present		1A , 1A'	Expression was detected in the rete cord. The expressing cells at the rete cord were found to be attached to the epithelial cells of the mesonephric tubules.
TS23: mesonephros		Present		1A' , 1A

Structure	Level	Pattern	Image	Note
TS23: ovary	Present		1C' , 1C	Expression was adjacent to the epithelial cells of the mesonephric tubules.
TS23: mesonephros	Present		1C , 1C'

Structure	Level	Pattern	Image	Note
TS19: urogenital ridge	Present	Regionally restricted	2A , 2A'	Expression was detected in somatic cells of the developing gonadal region.
TS19: genitourinary system	Present	Regionally restricted	2A , 2A'	Expression was detected in cells in the boundary region between the genital ridge and mesonephros.

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Present	Regionally restricted	3A , 3A'	Expression was detected in the coelomic epithelium of the mesonephros.
TS17: mesonephros mesenchyme	Present	Regionally restricted	3A' , 3A	Some mesenchymal cells under the coelomic epithelium were immunopositive, and some were located adjacent to the mesonephric tubules.

Structure	Level	Pattern	Image	Note
TS15: mesonephros	Present	Regionally restricted	4A , 4A'	Expression was detected in the coelomic epithelium of the mesonephros.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: Leydig cell	Leydig cell	Present		5B
TS23: Sertoli cell	Sertoli cell	Present		5B
TS23: rete testis		Present		5B	Expression was detected in the rete cord adjacent to the mesonephric tubules.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: Sertoli cell	Sertoli cell	Present		5F
TS20: mesonephros mesenchyme		Present		5F	Expression was detected in cells adjacent to the mesonephric tubules.

Structure	Level	Pattern	Image	Note
TS19: mesonephros	Present	Regionally restricted	5J	Expression was detected in cells adjacent to the mesonephric tubules.

Structure	Level	Pattern	Image	Note
TS19: mesonephros	Present	Regionally restricted	5M	Expression was detected in cells adjacent to the mesonephric tubules.