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Gene Expression Data
Assay Details
Assay
Reference: J:248983 Varadarajan SG, et al., Netrin1 establishes multiple boundaries for axon growth in the developing spinal cord. Dev Biol. 2017 Oct 1;430(1):177-187
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6446838
Gene symbol: Ntn1
Gene name: netrin 1
Reporter: lacZ
Antibody: anti-B-galactosidase
Results
Specimen 2C: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 2C Expression in two distinct regions of spinal cord. Expression excluded from dorsal-most spinal cord.
TS17: neural tube floor plate Strong 2C
TS17: neural tube lateral wall Weak Regionally restricted 2C Lower level of expression by neural progenitor cells in ventricular zone.
Specimen 2E: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Ntn1 reporter; green - Olig2 (assay MGI:6446813); blue - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 2E Expression excluded from Olig2-expressing motor neuron progenitors and early post-mitotic column.
Specimen 2E': embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Ntn1 reporter; green - Olig2 (assay MGI:6446813); blue - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 2E' beta gal , 2E' Expression excluded from Olig2-expressing motor neuron progenitors and early post-mitotic column in lumbar sections.
Specimen 2F: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Ntn1 reporter; green - Olig2 (assay MGI:6446813); blue - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 2F beta gal , 2F Expression excluded from Olig2-expressing motor neuron progenitors and early post-mitotic column in thoracic sections,
Specimen 2I: embryonic day 11.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present Graded 2I Broad domain of graded expression in ventricular zone, extending from floor plate into dorsal spinal cord, ending at dorsal root entry zone (DREZ).
Specimen 2M: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Structure Level Pattern Image Note
TS20: spinal cord ventricular layer Present Graded 2M Bilaterally symmetric domains of graded expression in ventricular zone.
TS20: spinal cord lateral wall Present Regionally restricted 2M Additional domain of expression in lateral region, immediately adjacent to dorsal root entry zone (DREZ).
Specimen 3A: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Double labeled: red - Ntn1 reporter; green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3A
Specimen 3B: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Double labeled: red - Ntn1 reporter; green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3B Dorsal-most border of expression coincident with position of ventrally-directed axons growth.
Specimen 3C: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Double labeled: red - Ntn1 reporter; green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3C Expression boundary in ventricular maintained.
Specimen 3D: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Double labeled: red - Ntn1 reporter; green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3D Dorsal-most border of expression coincident with position of ventrally-directed axons growth.
Specimen 3G: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Double labeled: red - Ntn1 reporter; green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3G
Specimen 3H: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Double labeled: red - Ntn1 reporter; green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3H Dorsal boundary of expression.
Specimen 3I: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Double labeled: red - Ntn1 reporter; green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3I
Specimen 3J: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Double labeled: red - Ntn1 reporter; green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3J Dorsal boundary of expression.
Specimen 4A: embryonic day 11.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Ntn1 reporter; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS19: neural tube floor plate Present 4A beta gal , 4A
Specimen 4C: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Ntn1 reporter; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord ventricular layer Present Regionally restricted 4C Expression boundary in ventriclar zone maintained in E12.5 embryos.
Specimen 4D: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Ntn1 reporter; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Present Regionally restricted 4D beta gal , 4D Border of expression spanning from dorsal ventricular zone to apical floor plate.
Specimen 4E: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Ntn1 reporter; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Present Regionally restricted 4E Border of expression spanning from dorsal ventricular zone to apical floor plate.
Specimen 4F: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Ntn1 reporter; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord floor plate Present Regionally restricted 4F beta gal , 4F Border of expression spanning from dorsal ventricular zone to apical floor plate.
Specimen 4G: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Double labeled: red - Ntn1; green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord ventricular layer Present Regionally restricted 4G , 4G beta gal Border of expression spanning from dorsal ventricular zone to apical floor plate.
Specimen 4H: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Double labeled: red - Ntn1; green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Present Regionally restricted 4H , 4H beta gal Expression domain in dorsal root entry zone (DREZ).
Specimen 5A: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Double labeled: red - Ntn1; green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Present Regionally restricted 5A Lateral expression domain below dorsal root entry zone (DREZ).
Specimen 5B/E: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Double labeled: red - Ntn1; green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Present Regionally restricted 5E , 5B Lateral expression domain below dorsal root entry zone (DREZ).
Specimen 5F: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Double labeled: red - Ntn1; green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present 5F
Specimen 5G/J: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Double labeled: red - Ntn1; green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present 5G , 5J
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/22/2024
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