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Gene Expression Data
Assay Details
Assay
Reference: J:248983 Varadarajan SG, et al., Netrin1 establishes multiple boundaries for axon growth in the developing spinal cord. Dev Biol. 2017 Oct 1;430(1):177-187
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6446810
Gene symbol: Robo3
Gene name: roundabout guidance receptor 3
Results
Specimen 1D: embryonic day 10.5 (more )
Note: Triple labeled: red - Robo3; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 1D Expression in axon projects of commissural axons avoiding ventricular zone.
Specimen 1E: embryonic day 10.5 (more )
Note: Triple labeled: red - Robo3; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 1E , 1E Robo3 Expression in axons in mantle zone.
Specimen 1I: embryonic day 11.5 (more )
Note: Triple labeled: red - Robo3; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 1I Expression in spinal axons avoiding ventricular zone.
TS19: neural tube ventricular layer Absent 1I
TS19: neural tube floor plate Present 1I Expression in commissural axons completing their trajectory to floor plate at ventral midline.
Specimen 1J: embryonic day 11.5 (more )
Note: Triple labeled: red - Robo3; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 1J , 1J Robo3 Expression in spinal axons avoiding ventricular zone.
TS19: neural tube ventricular layer Absent 1J , 1J Robo3
Specimen 1N: embryonic day 12.5 (more )
Note: Triple labeled: red - Robo3; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord thoracic region Present Regionally restricted 1N Expression in spinal axons avoiding ventricular zone.
TS20: spinal cord ventricular layer Absent 1N
TS20: spinal cord floor plate Present 1N Expression in commissural axons completing their trajectory to floor plate at ventral midline.
Specimen 1O: embryonic day 12.5 (more )
Note: Triple labeled: red - Robo3; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Present Regionally restricted 1O , 1O Robo3 Expression in spinal axons avoiding ventricular zone.
TS20: spinal cord ventricular layer Absent 1O Robo3 , 1O
Specimen 3K/N: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Robo3; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3N , 3K Expression in commissural axons pioneering their path to floor plate at ventral midline.
Specimen 3L: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Robo3; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3L Expression in commissural axons pioneering their path to floor plate at ventral midline.
Specimen 3P/S: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: red - Robo3; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3P , 3S Expression in axons projecting randomly along dorsal-ventral axis of spinal cord.
Specimen 3Q: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: red - Robo3; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3Q Expression in axons projecting randomly along dorsal-ventral axis of spinal cord.
Specimen 5K/O: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Robo3; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5K , 5O Expression in axons forming two major fascicles.
TS20: spinal cord ventricular layer Absent 5K , 5O Expression in axons projecting precisely around the ventricular zone.
Specimen 5L: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Robo3; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5L (bottom) , 5L Expression domain adjacent to ventricular zone.
Specimen 5M: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: red - Robo3; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5M Robo3 , 5M Expression domain adjacent to ventricular zone.
Specimen 5P/T: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: red - Robo3; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5P , 5T Expression in axons continuing to extend robustly into the ventricular zone.
Specimen 5Q: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: red - Robo3; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5Q (bottom) , 5Q Expression in axons continuing to extend robustly into the ventricular zone.
Specimen 5R: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: red - Robo3; blue - Cntn2 (assay MGI:6446808); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5R Robo3 , 5R Expression in fascicles that are almost completely defasciculated.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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