About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:248983 Varadarajan SG, et al., Netrin1 establishes multiple boundaries for axon growth in the developing spinal cord. Dev Biol. 2017 Oct 1;430(1):177-187
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6446808
Gene symbol: Cntn2
Gene name: contactin 2
Results
Specimen 1A: embryonic day 10.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 1A Expression in axon projects of commissural axons avoiding ventricular zone.
Specimen 1B: embryonic day 10.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 1B Tag1 , 1B Expression in axons in mantle zone.
Specimen 1F: embryonic day 11.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 1F Expression in spinal axons avoiding ventricular zone.
TS19: neural tube ventricular layer Absent 1F
TS19: neural tube floor plate Present 1F Expression in commissural axons completing their trajectory to floor plate at ventral midline.
Specimen 1G: embryonic day 11.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 1G , 1G Tag1 Expression in spinal axons avoiding ventricular zone.
TS19: neural tube ventricular layer Absent 1G Tag1 , 1G
Specimen 1K: embryonic day 12.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 1K Expression in spinal axons avoiding ventricular zone.
TS20: spinal cord ventricular layer Absent 1K
TS20: spinal cord floor plate Present 1K Expression in commissural axons completing their trajectory to floor plate at ventral midline.
Specimen 1L: embryonic day 12.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Present Regionally restricted 1L Tag1 , 1L Expression in commissural axons completing their trajectory to floor plate at ventral midline.
TS20: spinal cord ventricular layer Absent 1L , 1L Tag1
Specimen 3K/O: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3O , 3K Expression in commissural axons pioneering their path to floor plate at ventral midline.
TS17: dorsal root ganglion Present 3K , 3O
Specimen 3L: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3L Expression in motor columns.
Specimen 3P/T: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3T , 3P Expression in commissural axons showing an axons growth defect.
TS17: dorsal root ganglion Present 3T , 3P
Specimen 3Q: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3Q
Specimen 4A: embryonic day 11.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Ntn1 reporter (assay MGI:6446838); green - neurofilament;.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4A (top right) , 4A Expression in axons projecting across floor plate at ventral midline extending under Ntn1 expression domain in floor plate cells.
Specimen 4B: embryonic day 11.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Ntn1 protein (assay MGI:6446811); green - neurofilament;.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4B (top right) , 4B Expression in axons projecting across floor plate at ventral midline extending under Ntn1 expression domain in floor plate cells.
Specimen 4E: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Ntn1 reporter (assay MGI:6446838); green - neurofilament;.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 4E Expression in axons projecting specifically within domain of lowest Ntn1 expression. Expression in axins projecting precisely around the ventricular zone.
Specimen 4F: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Ntn1 reporter (assay MGI:6446838); green - neurofilament;.
Structure Level Pattern Image Note
TS20: spinal cord floor plate Present Regionally restricted 4F , 4F (bottom) Expression in axons growing around a continuous border of Ntn1-expressing cells.
Specimen 5K: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5K Expression in axons forming two major fascicles:
Specimen 5L: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5L Expression domain adjacent to ventricular zone.
Specimen 5M: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5M
Specimen 5P: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5P Expression in axons continuing to extend robustly into the ventricular zone.
Specimen 5Q: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5Q Expression in axons continuing to extend robustly into the ventricular zone.
Specimen 5R: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5R Expression in fascicles that are almost completely defasciculated.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory