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Gene Expression Data
Assay Details
Assay
Reference: J:248983 Varadarajan SG, et al., Netrin1 establishes multiple boundaries for axon growth in the developing spinal cord. Dev Biol. 2017 Oct 1;430(1):177-187
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6446808
Gene symbol: Cntn2
Gene name: contactin 2
Results
Specimen 1A: embryonic day 10.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 1A Expression in axon projects of commissural axons avoiding ventricular zone.
Specimen 1B: embryonic day 10.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 1B Tag1 , 1B Expression in axons in mantle zone.
Specimen 1F: embryonic day 11.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 1F Expression in spinal axons avoiding ventricular zone.
TS19: neural tube ventricular layer Absent 1F
TS19: neural tube floor plate Present 1F Expression in commissural axons completing their trajectory to floor plate at ventral midline.
Specimen 1G: embryonic day 11.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 1G , 1G Tag1 Expression in spinal axons avoiding ventricular zone.
TS19: neural tube ventricular layer Absent 1G Tag1 , 1G
Specimen 1K: embryonic day 12.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 1K Expression in spinal axons avoiding ventricular zone.
TS20: spinal cord ventricular layer Absent 1K
TS20: spinal cord floor plate Present 1K Expression in commissural axons completing their trajectory to floor plate at ventral midline.
Specimen 1L: embryonic day 12.5 (more )
Note: Triple labeled: red - Cntn2; blue - Sox2 (assay MGI:6446809); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord lateral wall Present Regionally restricted 1L Tag1 , 1L Expression in commissural axons completing their trajectory to floor plate at ventral midline.
TS20: spinal cord ventricular layer Absent 1L , 1L Tag1
Specimen 3K/O: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3O , 3K Expression in commissural axons pioneering their path to floor plate at ventral midline.
TS17: dorsal root ganglion Present 3K , 3O
Specimen 3L: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3L Expression in motor columns.
Specimen 3P/T: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3T , 3P Expression in commissural axons showing an axons growth defect.
TS17: dorsal root ganglion Present 3T , 3P
Specimen 3Q: embryonic day 10.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3Q
Specimen 4A: embryonic day 11.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Ntn1 reporter (assay MGI:6446838); green - neurofilament;.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4A (top right) , 4A Expression in axons projecting across floor plate at ventral midline extending under Ntn1 expression domain in floor plate cells.
Specimen 4B: embryonic day 11.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Ntn1 protein (assay MGI:6446811); green - neurofilament;.
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4B (top right) , 4B Expression in axons projecting across floor plate at ventral midline extending under Ntn1 expression domain in floor plate cells.
Specimen 4E: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Ntn1 reporter (assay MGI:6446838); green - neurofilament;.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 4E Expression in axons projecting specifically within domain of lowest Ntn1 expression. Expression in axins projecting precisely around the ventricular zone.
Specimen 4F: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Ntn1 reporter (assay MGI:6446838); green - neurofilament;.
Structure Level Pattern Image Note
TS20: spinal cord floor plate Present Regionally restricted 4F , 4F (bottom) Expression in axons growing around a continuous border of Ntn1-expressing cells.
Specimen 5K: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5K Expression in axons forming two major fascicles:
Specimen 5L: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5L Expression domain adjacent to ventricular zone.
Specimen 5M: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1+ (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5M
Specimen 5P: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5P Expression in axons continuing to extend robustly into the ventricular zone.
Specimen 5Q: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5Q Expression in axons continuing to extend robustly into the ventricular zone.
Specimen 5R: embryonic day 12.5; Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg (more )
Note: Triple labeled: blue - Cntn2; red - Robo3 (assay MGI:6446810); green - neurofilament.
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 5R Expression in fascicles that are almost completely defasciculated.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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