Gene Expression Data
Assay Details
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Assay
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Reference:
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J:49643
Gottlieb S, et al., Goosecoid-like, a gene deleted in DiGeorge and velocardiofacial syndromes, recognizes DNA with a bicoid-like specificity and is expressed in the developing mouse brain. Hum Mol Genet. 1998 Sep;7(9):1497-505
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6445542
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Gene symbol:
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Gsc2
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Gene name:
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goosecoid homebox 2
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Probe:
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Gscl cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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The authors state two different probes were used that gave identical results and did not specify which one was used. It is possible that Gscl cDNA2 was used (MGI:6445521).
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Results
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Specimen
3A:
embryonic day 9.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: diencephalon |
Present |
Regionally restricted |
3A
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Expression is as a narrow band in the diencephalon and was absent everywhere else in the embryo.
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Specimen
3B:
embryonic day 11.5
(more )
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
3B
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Expression is dorsal in the region of the prospective thalamus and epithalamus.
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| TS19: hindbrain |
Present |
Regionally restricted |
3B
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Expression is in the anterior hindbrain.
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| TS19: metencephalon basal plate |
Present |
Regionally restricted |
3B
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Expression is punctate in the prospective pons area.
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| TS19: prethalamus |
Present |
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3B
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The authors state that there was no detectable expression in tissues outside of the brain.
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Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: habenula |
Present |
Regionally restricted |
3C
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Expression is in the ventricular zone.
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Specimen
3D:
embryonic day 12.5
(more )
Specimen
3D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pons |
Strong |
Regionally restricted |
3D
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Expression is in the region of the serotonergic nuclear complex.
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Specimen
4A:
embryonic day 12.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: epithalamus |
Present |
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4A
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The authors state that there was no detectable expression in tissues outside of the brain.
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Specimen
5A:
embryonic day 15.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pons |
Strong |
Regionally restricted |
5A
,
5B
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Expression is in the region of the dorsal and median raphe nuclei.
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| TS23: epithalamus |
Absent |
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5B
,
5A
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Specimen
6A:
postnatal adult
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown E10.5:
embryonic day 10.5
(more )
Specimen
not shown E10.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: diencephalon |
Present |
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The authors state that there was no detectable expression in tissues outside of the brain.
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| TS17: hindbrain |
Weak |
Regionally restricted |
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Expression is in the anterior hindbrain.
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Analysis Tools