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Gene Expression Data
Assay Details
Assay
Reference: J:231312 Baardman ME, et al., Common arterial trunk and ventricular non-compaction in Lrp2 knockout mice indicate a crucial role of LRP2 in cardiac development. Dis Model Mech. 2016 Apr 1;9(4):413-25
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6444460
Gene symbol: Nkx2-5
Gene name: NK2 homeobox 5
Antibody: anti-Nkx-2.5 (N-19): sc-8697
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488
Results
Specimen 2A: embryonic day 10.5 (more )
Note: Double labeled: green - Nkx2-5; red - Lrp2 (assay MGI:6444467).
Structure Level Pattern Image Note
TS17: cardiac muscle tissue Present 2A
TS17: secondary heart field Present 2A
Specimen 2B: embryonic day 11.5 (more )
Note: Double labeled: green - Nkx2-5; red - Lrp2 (assay MGI:6444467).
Structure Level Pattern Image Note
TS19: cardiac muscle tissue Present 2B
Specimen 2C: embryonic day 12.5 (more )
Note: Double labeled: green - Nkx2-5; red - Lrp2 (assay MGI:6444467).
Structure Level Pattern Image Note
TS20: cardiac muscle tissue Present 2C
Specimen 2D: embryonic day 13.5 (more )
Note: Double labeled: green - Nkx2-5; red - Lrp2 (assay MGI:6444467).
Structure Level Pattern Image Note
TS21: cardiac muscle tissue Present 2D
Specimen 3A: embryonic day 10.5 (more )
Note: Double labeled: green - Nkx2-5; red - Tfap2a (assay MGI:6444470).
Structure Level Pattern Image Note
TS17: cardiac muscle tissue Present 3A Tfap2a and Nkx2-5 were not co-expressed.
TS17: secondary heart field Present 3A Tfap2a and Nkx2-5 were not co-expressed.
Specimen 3D: embryonic day 10.5; Lrp2tm1Her/Lrp2tm1Her (more )
Note: Double labeled: green - Nkx2-5; red - Tfap2a (assay MGI:6444470).
Structure Level Pattern Image Note
TS17: cardiac muscle tissue Present 3D Tfap2a and Nkx2-5 were not co-expressed.
TS17: secondary heart field Present 3D Tfap2a and Nkx2-5 were not co-expressed.
Specimen 3B/C: embryonic day 11.5 (more )
Note: Double labeled: green - Nkx2-5; red - Tfap2a (assay MGI:6444470).
Structure Level Pattern Image Note
TS19: cardiac muscle tissue Present 3B , 3C Tfap2a and Nkx2-5 were not co-expressed. The flow divider was a part of the Nkx2-5-positive cell population and was present in between the most caudal pharyngeal arch arteries.
Specimen 3E/F: embryonic day 11.5; Lrp2tm1Her/Lrp2tm1Her (more )
Note: Double labeled: green - Nkx2-5; red - Tfap2a (assay MGI:6444470).
Structure Level Pattern Image Note
TS19: cardiac muscle tissue Present 3E , 3F Tfap2a and Nkx2-5 were not co-expressed. The flow divider was a part of the Nkx2-5-positive cell population and was present in between the most caudal pharyngeal arch arteries.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory