Gene Expression Data
Assay Details
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Assay
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Reference:
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J:265803
Da Silva-Alverez S, et al., Pkd2 deletion during embryo development does not alter mesonephric programmed cell senescence. Int J Dev Biol. 2018;62(9-10):637-640
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6441151
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Gene symbol:
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Pkd2
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Gene name:
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polycystin 2, transient receptor potential cation channel
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixation with 2% formaldehyde/0.2% glutaraldehyde.
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Results
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Specimen
1 top left:
(close  )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm1Som/Pkd2tm2Som
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: embryo |
Present |
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1_top left
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Specimen
1 top right:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm1Som/Pkd2tm2Som
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
1 bottom left:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm1Som/Pkd2tm2Som
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
1_bottom left
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Expression in closing neural tube.
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Specimen
1 bottom right:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm1Som/Pkd2tm2Som
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: vibrissa |
Present |
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1_bottom right
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Specimen
2A WT E13.5 Ki67:
embryonic day 13.5; Pkd2tm2Som/Pkd2 +
(more  )
Note: Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
Specimen
2A WT E13.5 Ki67:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm2Som/Pkd2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
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Specimen
2A WT E13.5 p21Cip1:
embryonic day 13.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
Specimen
2A WT E13.5 p21Cip1:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm2Som/Pkd2+
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
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Specimen
2A Pkd2-null E13.5 Ki67:
embryonic day 13.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
Specimen
2A Pkd2-null E13.5 Ki67:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm1Som/Pkd2tm2Som
|
|
Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: endolymphatic sac |
Strong |
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2A Pkd2-null E13.5 Ki67
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Exact same pattern as in wild type embryos.
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Specimen
2A Pkd2-null E13.5 p21Cip1:
embryonic day 13.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
Specimen
2A Pkd2-null E13.5 p21Cip1:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm1Som/Pkd2tm2Som
|
|
Age:
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embryonic day 13.5
|
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: endolymphatic sac |
Strong |
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2A Pkd2-null E13.5 P21Cip1
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Exact same pattern as in wild type embryos.
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Specimen
2A WT E14.5 Ki67:
embryonic day 14.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
Specimen
2A WT E14.5 Ki67:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Pkd2tm2Som/Pkd2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
|
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Embedding:
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Paraffin
|
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Note:
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Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
|
Specimen
2A WT E14.5 p21Cip1:
embryonic day 14.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
Specimen
2A WT E14.5 p21Cip1:
(close )
|
Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
|
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Mutant Allele(s):
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Pkd2tm2Som/Pkd2+
|
|
Age:
|
embryonic day 14.5
|
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Sex:
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Not Specified
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Type:
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section from whole mount
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|
Fixation:
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Other - See Notes
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|
Embedding:
|
Paraffin
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|
Note:
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Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
|
Specimen
2A Pkd2-null E14.5 Ki67:
embryonic day 14.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
Specimen
2A Pkd2-null E14.5 Ki67:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Pkd2tm1Som/Pkd2tm2Som
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: endolymphatic sac |
Strong |
|
2A Pkd2-null E14.5 Ki67
|
Exact same pattern as in wild type embryos.
|
Specimen
2A Pkd2-null E14.5 p21Cip1:
embryonic day 14.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
Specimen
2A Pkd2-null E14.5 p21Cip1:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Pkd2tm1Som/Pkd2tm2Som
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
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Not Specified
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Type:
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section from whole mount
|
|
Fixation:
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Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
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Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: endolymphatic sac |
Strong |
|
2A Pkd2-null E14.5 p21Cip1
|
Exact same pattern as in wild type embryos.
|
Specimen
2B WT E13.5 Ki67:
embryonic day 13.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
Specimen
2B WT E13.5 Ki67:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Pkd2tm2Som/Pkd2+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
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Not Specified
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Type:
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section from whole mount
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|
Fixation:
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Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
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Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mesonephric tubule |
Weak |
Regionally restricted |
2B WT E13.5 Ki67
|
Pale and punctuated blue dots surround cells that ae part of tubules.
|
Specimen
2B WT E13.5 p21Cip1:
embryonic day 13.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
Specimen
2B WT E13.5 p21Cip1:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Pkd2tm2Som/Pkd2+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mesonephric tubule |
Weak |
Regionally restricted |
2B WT E13.5 p21Cip1
|
Pale and punctuated blue dots surround cells that ae part of tubules.
|
Specimen
2B Pkd2-null E13.5 Ki67:
embryonic day 13.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
Specimen
2B Pkd2-null E13.5 Ki67:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Pkd2tm1Som/Pkd2tm2Som
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mesonephric tubule |
Weak |
Regionally restricted |
2B Pkd2-null E13.5 Ki67
|
Exact same pattern of light but clear expression as in wild type embryos
|
Specimen
2B Pkd2-null E13.5 p21Cip1:
embryonic day 13.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
Specimen
2B Pkd2-null E13.5 p21Cip1:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Pkd2tm1Som/Pkd2tm2Som
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mesonephric tubule |
Weak |
Regionally restricted |
2B Pkd2-null E13.5 p21Cip1
|
Exact same pattern of light but clear expression as in wild type embryos
|
Specimen
2B WT E14.5 Ki67:
embryonic day 14.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
Specimen
2B WT E14.5 Ki67:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Pkd2tm2Som/Pkd2+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesonephric tubule |
Weak |
Regionally restricted |
2B WT E14.5 Ki67
|
Pale and punctuated blue dots surround cells that ae part of tubules.
|
Specimen
2B WT E14.5 p21Cip1:
embryonic day 14.5; Pkd2tm2Som/Pkd2 +
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
Specimen
2B WT E14.5 p21Cip1:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Pkd2tm2Som/Pkd2+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesonephric tubule |
Weak |
Regionally restricted |
2B WT E14.5 p21Cip1
|
Pale and punctuated blue dots surround cells that ae part of tubules.
|
Specimen
2B Pkd2-null E14.5 Ki67:
embryonic day 14.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
Specimen
2B Pkd2-null E14.5 Ki67:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Pkd2tm1Som/Pkd2tm2Som
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - Pkd2 reporter; brown - Mki67 (assay MGI:6441157).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesonephric tubule |
Weak |
Regionally restricted |
2B Pkd2-null E14.5 Ki67
|
Exact same pattern of light but clear expression as in wild type embryos
|
Specimen
2B Pkd2-null E14.5 p21Cip1:
embryonic day 14.5; Pkd2tm1Som/ Pkd2tm2Som
(more )
Note: Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
Specimen
2B Pkd2-null E14.5 p21Cip1:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Pkd2tm1Som/Pkd2tm2Som
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - Pkd2 reporter; brown - p21Cip1 (Cdkn1a assay MGI:6441158).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesonephric tubule |
Weak |
Regionally restricted |
2B Pkd2-null E14.5 p21Cip1
|
Exact same pattern of light but clear expression as in wild type embryos
|
|
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