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Gene Expression Data
Assay Details
Assay
Reference: J:265803 Da Silva-Alverez S, et al., Pkd2 deletion during embryo development does not alter mesonephric programmed cell senescence. Int J Dev Biol. 2018;62(9-10):637-640
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6441158
Gene symbol: Cdkn1a
Gene name: cyclin dependent kinase inhibitor 1A
Antibody: anti-p21 (HUGO-291; ab107099)
Detection system: horseradish peroxidase
Assay notes: Fixation with 2% formaldehyde/0.2% glutaraldehyde.
Results
Specimen 2A WT E13.5 p21Cip1: embryonic day 13.5; Pkd2tm2Som/Pkd2+ (more )
Note: Double labeled: brown - p21Cip1 (Cdkn1a); blue - Pkd2 reporter (assay MGI:6441151).
Structure Level Pattern Image Note
TS21: endolymphatic sac Present 2A WT E13.5 p21Cip1
Specimen 2A Pkd2-null E13.5 p21Cip1: embryonic day 13.5; Pkd2tm1Som/Pkd2tm2Som (more )
Note: Double labeled: brown - p21Cip1 (Cdkn1a); blue - Pkd2 reporter (assay MGI:6441151).
Structure Level Pattern Image Note
TS21: endolymphatic sac Present 2A Pkd2-null E13.5 P21Cip1 Exact same pattern as in wild type embryos.
Specimen 2A WT E14.5 p21Cip1: embryonic day 14.5; Pkd2tm2Som/Pkd2+ (more )
Note: Double labeled: brown - p21Cip1 (Cdkn1a); blue - Pkd2 reporter (assay MGI:6441151).
Structure Level Pattern Image Note
TS22: endolymphatic sac Present 2A WT E14.5 p21Cip1
Specimen 2A Pkd2-null E14.5 p21Cip1: embryonic day 14.5; Pkd2tm1Som/Pkd2tm2Som (more )
Note: Double labeled: brown - p21Cip1 (Cdkn1a); blue - Pkd2 reporter (assay MGI:6441151).
Structure Level Pattern Image Note
TS22: endolymphatic sac Present 2A Pkd2-null E14.5 p21Cip1 Exact same pattern as in wild type embryos.
Specimen 2B WT E13.5 p21Cip1: embryonic day 13.5; Pkd2tm2Som/Pkd2+ (more )
Note: Double labeled: brown - p21Cip1 (Cdkn1a); blue - Pkd2 reporter (assay MGI:6441151).
Structure Level Pattern Image Note
TS21: mesonephric tubule Present 2B WT E13.5 p21Cip1
Specimen 2B Pkd2-null E13.5 p21Cip1: embryonic day 13.5; Pkd2tm1Som/Pkd2tm2Som (more )
Note: Double labeled: brown - p21Cip1 (Cdkn1a); blue - Pkd2 reporter (assay MGI:6441151).
Structure Level Pattern Image Note
TS21: mesonephric tubule Present 2B Pkd2-null E13.5 p21Cip1 Exact same pattern as in wild type embryos.
Specimen 2B WT E14.5 p21Cip1: embryonic day 14.5; Pkd2tm2Som/Pkd2+ (more )
Note: Double labeled: brown - p21Cip1 (Cdkn1a); blue - Pkd2 reporter (assay MGI:6441151).
Structure Level Pattern Image Note
TS22: mesonephric tubule Present 2B WT E14.5 p21Cip1
Specimen 2B Pkd2-null E14.5 p21Cip1: embryonic day 14.5; Pkd2tm1Som/Pkd2tm2Som (more )
Note: Double labeled: brown - p21Cip1 (Cdkn1a); blue - Pkd2 reporter (assay MGI:6441151).
Structure Level Pattern Image Note
TS22: mesonephric tubule Present 2B Pkd2-null E14.5 p21Cip1 Exact same pattern as in wild type embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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