Probe:	Hdac1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS16: branchial arch	Strong		5A
TS16: otocyst	Strong		5A
TS16: telencephalon	Strong		5A
TS16: diencephalon	Present		5A
TS16: future midbrain	Present		5A
TS16: future hindbrain	Present	Regionally restricted	5A	Expression is in the rhombic fossa and is weak in the myelencephalon.
TS16: future spinal cord	Present	Regionally restricted	5A	Expression is in the caudal region at the site of neural tube closure.
TS16: forelimb bud	Weak		5A
TS16: head mesenchyme	Weak		5A
TS16: hindlimb bud	Weak		5A

Structure	Level	Pattern	Image	Note
TS16: branchial arch	Strong		5B
TS16: otocyst	Strong		5B
TS16: telencephalon	Strong		5B
TS16: diencephalon	Present		5B
TS16: future midbrain	Present		5B
TS16: future hindbrain	Present	Regionally restricted	5B	Expression is in the rhombic fossa and is weak in the myelencephalon.
TS16: future spinal cord	Present	Regionally restricted	5B	Expression is in the caudal region at the site of neural tube closure.
TS16: forelimb bud	Weak		5B
TS16: head mesenchyme	Weak		5B
TS16: hindlimb bud	Weak		5B

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Strong		5C
TS19: diencephalon	Strong		5C
TS19: forelimb bud	Strong		5C
TS19: hindlimb bud	Strong		5C
TS19: metencephalon	Strong		5C
TS19: midbrain	Strong		5C
TS19: otocyst	Strong		5C
TS19: telencephalon	Strong		5C
TS19: hindbrain	Strong	Regionally restricted	5C	Expression is in the rhombic fossa.
TS19: myelencephalon	Weak	Regionally restricted	5C	Expression extends caudally as two faint stripes adjacent to the midline of the spinal cord.
TS19: neural tube	Present	Regionally restricted	5C	Expression is stronger in the caudal region at the site of neural tube closure.
TS19: dorsal root ganglion	Weak		5C
TS19: head mesenchyme	Weak		5C
TS19: liver	Weak		5C
TS19: somite	Weak		5C

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Strong		5D
TS19: forelimb bud	Strong		5D
TS19: hindlimb bud	Strong		5D
TS19: metencephalon	Strong		5D
TS19: otocyst	Strong		5D
TS19: telencephalon	Strong		5D
TS19: hindbrain	Strong	Regionally restricted	5D	Expression is in the rhombic fossa.
TS19: myelencephalon	Weak	Regionally restricted	5D	Expression extends caudally as two faint stripes adjacent to the midline of the spinal cord.
TS19: neural tube	Present	Regionally restricted	5D	Expression is stronger in the caudal region at the site of neural tube closure.
TS19: dorsal root ganglion	Weak		5D
TS19: head mesenchyme	Weak		5D
TS19: somite	Weak		5D

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Strong		5E
TS21: diencephalon	Strong		5E
TS21: midbrain	Strong		5E
TS21: telencephalon	Strong		5E
TS21: hindbrain	Strong	Regionally restricted	5E	Expression is in the cerebellum and is absent in the rhombic fossa.
TS21: spinal cord	Present	Regionally restricted	5E	Expression extends caudally as two stripes adjacent to the midline of the spinal cord.
TS21: oral region	Present	Regionally restricted	5E	Expression is in the mandibular and maxillary processes.
TS21: dorsal root ganglion	Present		5E
TS21: eye	Present		5E
TS21: eyelid	Present		5E
TS21: genital tubercle	Present		5E
TS21: philtrum	Present		5E
TS21: somite	Present		5E
TS21: vibrissa	Present		5E
TS21: forelimb mesenchyme	Present	Regionally restricted	5E	Expression is concentrated around the skeletal elements.
TS21: hindlimb mesenchyme	Present	Regionally restricted	5E	Expression is concentrated around the skeletal elements.
TS21: inner ear	Present	Regionally restricted	5E	Expression is in the otic vesicles.
TS21: forelimb cartilage condensation	Absent		5E
TS21: hindlimb cartilage condensation	Absent		5E

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Strong		5F
TS21: midbrain	Strong		5F
TS21: hindbrain	Strong	Regionally restricted	5F	Expression is in the cerebellum and is absent in the rhombic fossa.
TS21: spinal cord	Present	Regionally restricted	5F	Expression extends caudally as two stripes adjacent to the midline of the spinal cord.

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present	Regionally restricted	5X	Expression is in the ependyma layer.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Strong		6B
TS21: cerebral cortex mantle layer	Strong		6B
TS21: hindbrain	Strong	Regionally restricted	6B	Expression is in the cerebellum and is absent in the rhombic fossa.
TS21: choroid plexus	Absent		6B

Structure	Level	Pattern	Image	Note
TS21: eyelid	Present		6C
TS21: retina	Present		6C

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex mantle layer	Present		6D

Structure	Level	Pattern	Image	Note
TS21: nasal cavity	Present		6I
TS21: tooth	Present		6I
TS21: vibrissa	Present		6I

Structure	Level	Pattern	Image	Note
TS21: metanephros	Strong		6M
TS21: dorsal root ganglion	Present		6M
TS21: rib cartilage condensation	Present		6M
TS21: vertebral cartilage condensation	Present		6M

Structure	Level	Pattern	Image	Note
TS21: metanephros	Strong		6Q
TS21: adrenal gland	Present		6Q
TS21: gonad	Present		6Q

Structure	Level	Pattern	Image	Note
TS21: lung	Strong		6R
TS21: liver	Present	Regionally restricted	6R	Expression is in the capsule and the subcapsular region.
TS21: cardiac muscle tissue	Absent		6R

Structure	Level	Pattern	Image	Note
TS21: outflow tract	Present