Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS11: allantois	Present		6A	Expression throughout allantois and visceral yolk sac endoderm.
TS11: yolk sac endoderm	Present		6A	Expression throughout allantois and visceral yolk sac endoderm.

Structure	Level	Pattern	Image	Note
TS11: allantois	Strong	Regionally restricted	6B	Prominent expression throughout distal allantois, in both core and mesothelium.

Structure	Level	Pattern	Image	Note
TS11: Allantoic Core Domain	Present		6C	Relatively less intense expression in Allantoic Core Domain.
TS11: allantois-associated posterior visceral endoderm	Present	Regionally restricted	6C	Expression within allantois-associated posterior visceral endoderm near site of presumptive vessel of confluence.

Structure	Level	Pattern	Image	Note
TS11: allantois	Present	Regionally restricted	6D	Expression co-localizing with Runx1 within proximal allantois.

Structure	Level	Pattern	Image	Note
TS12: yolk sac endoderm	Present		6E
TS12: blood vessel	Present		6E	Expression in nascent vasculature and vitelline vessels.
TS12: vitelline blood vessel	Present		6E	Expression in nascent vasculature and vitelline vessels.
TS12: primitive streak	Absent		6E	No expression in primitive streak-associated cells adjacent to hindgut invagination.
TS12: primitive hindgut	Weak	Regionally restricted	6E	Faint colocalization with Mixl1 expression within ventral hindgut lip.
TS12: embryo mesoderm	Weak	Regionally restricted	6E	Faint colocalization with Mixl1 expression within proximo-ventral mesoderm.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Regionally restricted		Co-expression with Mixl1 in ventral allantoic mesothelium.

Structure	Level	Pattern	Image	Note
TS12: blood vessel	Present		6F	Expression in nascent vasculature and vitelline vessels.
TS12: vitelline blood vessel	Present		6F	Expression in nascent vasculature and vitelline vessels.
TS12: primitive streak	Absent		6F	No expression in primitive streak-associated cells adjacent to hindgut invagination.
TS12: primitive hindgut	Weak	Regionally restricted	6F	Faint colocalization with Mixl1 expression within ventral hindgut lip.
TS12: embryo mesoderm	Weak	Regionally restricted	6F	Faint colocalization with Mixl1 expression within proximo-ventral mesoderm.

Structure	Level	Pattern	Image	Note
TS12: blood vessel	Present		6G	Expression in nascent vasculature and vitelline vessels.
TS12: vitelline blood vessel	Present		6G	Expression in nascent vasculature and vitelline vessels.
TS12: primitive streak	Absent		6G	No expression in primitive streak-associated cells adjacent to hindgut invagination.
TS12: primitive hindgut	Weak	Regionally restricted	6G	Faint colocalization with Mixl1 expression within ventral hindgut lip.
TS12: embryo mesoderm	Weak	Regionally restricted	6G	Faint colocalization with Mixl1 expression within proximo-ventral mesoderm.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic endoderm	Present	Regionally restricted	6H	Expression colocalizing with Mixl1 expression in extraembryonic visceral endoderm.
TS13: blood island	Present	Regionally restricted	6H	Expression colocalizes with Mixl1 expression in presumptive erythroblasts.
TS13: vitelline artery	Present	Regionally restricted	6H	Expression localized to developing blood cells of the omphalomesenteric artery.
TS13: chorion ectoderm	Weak		6H	Expression to a lesser extent within lateral chorionic ectoderm.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic endoderm	Present	Regionally restricted	6I	Expression colocalizing with Mixl1 expression in extraembryonic visceral endoderm.
TS13: yolk sac	Present	Regionally restricted	6I	Within visceral yolk sac, co-localization with Mixl1 in both endothelium and blood cells.
TS13: blood island	Present	Regionally restricted	6I	Expression colocalizes with Mixl1 expression in presumptive erythroblasts. Stronger expression in blood cells.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic endoderm	Present	Regionally restricted	6J	Cololcalization with Mixl1 expression within extraembryonic visceral endoderm.
TS13: chorion ectoderm	Weak		6J	Expression to a lesser extent within lateral chorionic ectoderm.

Structure	Level	Pattern	Image	Note
TS13: embryo	Ambiguous		6K	Authors state sections were treated with brief X-gal exposure. No expression patterns were reported.

Structure	Level	Pattern	Image	Note
TS13: embryo	Ambiguous		6L	Authors state sections were treated with brief X-gal exposure. No expression patterns were reported.