Antibody:

anti-MIXl1

Structure	Level	Pattern	Image	Note
TS11: allantois	Strong		1E
TS11: posterior visceral endoderm	Strong		1E
TS11: primitive streak	Strong		1E

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Strong	Regionally restricted	1G	Strong expression in posterior primitive streak.
TS11: embryonic posterior visceral endoderm	Absent		1G	No expression in intraembryonic primitive streak-associated posterior visceral endoderm.

Structure	Level	Pattern	Image	Note
TS11: chorion ectoderm	Weak		1H
TS11: chorion mesoderm	Ambiguous		1H	Authors state there was no expression in individual cells in chorionic mesoderm and extraembryonic visceral endoderm.
TS11: extraembryonic visceral endoderm	Ambiguous		1H	Authors state there was no expression in individual cells in chorionic mesoderm and extraembryonic visceral endoderm.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Widespread	2A	Expression throughout primitive streak as it extended cranially.
TS10: embryo ectoderm	Absent		2A	No demonstrable expression in epiblast.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2B
TS10: embryo ectoderm	Absent		2B	No demonstrable expression in epiblast.
TS10: embryonic visceral endoderm	Present	Widespread	2B	Expression in throughout embryo-associated visceral endoderm at midstreak stage.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2C
TS10: embryo mesoderm	Present		2C	Expression in presumptive laterally-migrating embryonic mesoderm.
TS10: embryo ectoderm	Absent		2C	No demonstrable expression in epiblast.
TS10: embryonic visceral endoderm	Present	Regionally restricted	2C	Expression in embryo-associated visceral endoderm. Localization became biased toward posterior midline by late streak stage, with occasional expressing cells in anterior and lateral embryo-associated visceral endoderm.

Structure	Level	Pattern	Image	Note
TS10: extraembryonic primitive streak	Present	Widespread	2D	Expression in full-extent of primitive streak, including extraembryonic primitive streak that extends into the exocoelom.
TS10: primitive streak	Present	Widespread	2D	Expression in full-extent of primitive streak, including extraembryonic primitive streak that extends into the exocoelom.
TS10: node	Present	Regionally restricted	2D	Expression in ventral node and posterior crown.
TS10: posterior crown of node	Present	Regionally restricted	2D	Expression in ventral node and posterior crown.
TS10: allantois	Present	Widespread	2D	Expression in entire allantoic bud.
TS10: extraembryonic component	Present	Regionally restricted	2D	Expression in posterior chorion.

Structure	Level	Pattern	Image	Note
TS10: node	Strong	Regionally restricted	2E	Strong expression in ventral node and posterior crown of Hensen's node.
TS10: posterior crown of node	Strong	Regionally restricted	2E	Strong expression in ventral node and posterior crown of Hensen's node.

Structure	Level	Pattern	Image	Note
TS10: allantois-associated posterior visceral endoderm	Strong	Regionally restricted	2F
TS10: extraembryonic primitive streak	Strong	Regionally restricted	2F	Strong expression in adjacent extraembryonic primitive streak.

Structure	Level	Pattern	Image	Note
TS10: embryonic visceral endoderm	Present	Regionally restricted	2G	Expression in many cells of midline embryo-associated visceral endoderm.

Structure	Level	Pattern	Image	Note
TS11: allantois	Present	Regionally restricted	2H	Contiguous expression from primitive streak, extraembryonic primitive streak and allantoic bud.
TS11: primitive streak	Present	Regionally restricted	2H	Contiguous expression from primitive streak, extraembryonic primitive streak and allantoic bud.

Structure	Level	Pattern	Image	Note
TS11: allantois-associated posterior visceral endoderm	Strong		2I

Structure	Level	Pattern	Image	Note
TS11: embryonic posterior visceral endoderm	Present	Regionally restricted	2J	Intemittent expression at midline of intraembryonic primitive streak-associated visceral endoderm.

Structure	Level	Pattern	Image	Note
TS11: node	Present	Widespread	3A	Expression within ventral node. Some specimens demonstrate expression throughout ventral region.
TS11: posterior crown of node	Present		3A
TS11: primitive streak	Present	Regionally restricted	3A	Reduced breadth of expression in primitive streak. In some specimens, expression only in anterior and posterior ends of streak.
TS11: Allantoic Core Domain	Present		3A

Structure	Level	Pattern	Image	Note
TS11: posterior crown of node	Present		3B	Expression only within posterior crown.
TS11: node	Present	Regionally restricted	3B	Expression excluded from ventral node.
TS11: primitive streak	Present	Widespread	3B	Expression throughout primitive streak.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted		All streak-associated expression was ventrally localized except for posterior-most aspect at embryonic-extraembryonic junction where expression localized to full dorsal-ventral extent of streak.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	3C	Expression most prominently localized to posterior primitive streak.
TS11: Allantoic Core Domain	Strong	Regionally restricted	3C	Intense expression in ventral component of Allantoic Core Domain, adjacent to allantois-associated posterior visceral endoderm.
TS11: allantois-associated posterior visceral endoderm	Weak	Punctate	3C	Authors describe intermittent, punctate, faintly expressing cells within allantois-associated posterior visceral endoderm.
TS11: allantois	Weak	Single cells	3C	Punctate, faint expression in individual mesenchymal cells in distal allantois and overlying mesothelium.

Structure	Level	Pattern	Image	Note
TS11: Allantoic Core Domain	Present	Punctate	3G	Punctate nuclear expression in ventral Allantoic Core Domain.
TS11: allantois-associated posterior visceral endoderm	Present	Punctate	3G

Structure	Level	Pattern	Image	Note
TS11: Allantoic Core Domain	Present	Punctate	3G inset	Punctate nuclear expression in ventral Allantoic Core Domain.
TS11: allantois-associated posterior visceral endoderm	Present	Punctate	3G inset

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Weak		3J	Expression diminished in primitive streak and embryonic mesoderm.
TS11: primitive streak	Weak		3J	Expression diminished in primitive streak and embryonic mesoderm.
TS11: notochordal process	Absent		3J	Expression not appreciated in notochord.
TS11: embryonic visceral endoderm	Weak	Single cells	3J	Faint expression in cells of embryonic visceral endoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Weak		3J inset
TS11: primitive streak	Weak		3J inset
TS11: embryonic visceral endoderm	Weak	Single cells	3J inset	Faint expression in embryonic visceral endoderm toward anterior aspect of streak.

Structure	Level	Pattern	Image	Note
TS12: posterior crown of node	Strong		4A
TS12: primitive streak	Present	Regionally restricted	4A	Expression in posterior primitive streak. Occasional expressing cells within more anterior aspects of primitive streak.
TS12: Allantoic Core Domain	Present		4A

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Absent		4B	No expression in epiblast.
TS12: embryo mesoderm	Present	Regionally restricted	4B	No expression in embryonic mesoderm in this section but authors report expressng cells within mesoderm at midline posterior to node at each stage examined.
TS12: node	Present	Regionally restricted	4B	No expression in node in this section.

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Absent		4C	No expression in epiblast.
TS12: embryo mesoderm	Present	Regionally restricted	4C	No expression in embryonic mesoderm in this section but authors report expressng cells within mesoderm at midline posterior to node at each stage examined.
TS12: posterior crown of node	Present	Clusters	4C	Expression becomes evident and tightly clustered within posterior crown of the node.

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Absent		4D	No expression in epiblast
TS12: embryo mesoderm	Present	Regionally restricted	4D	No expression in embryonic mesoderm in this section but authors report expressng cells within mesoderm at midline posterior to node at each stage examined.
TS12: primitive streak	Present	Regionally restricted	4D	No expression in primitive streak immediately posterior to posterior crown of node.

Structure	Level	Pattern	Image	Note
TS12: posterior crown of node	Present	Regionally restricted	4E	Expression at midline expanding posteriorly from posterior crown of node.
TS12: embryo mesoderm	Present	Scattered	4E	Scattered expressing cells appearing within nascent embryonic mesoderm.

Structure	Level	Pattern	Image	Note
TS14: tail bud	Present	Clusters	5B	Expressing cells clustered within tailbud mesoderm adjacent to posterior end of hindgut.
TS14: notochord	Present	Regionally restricted	5B	Expression localizes to putative posterior notochord population of axial mesodermal cells.
TS14: vitelline artery	Absent		5B	Expression excluded from omphalomesenteric artery.
TS14: primitive hindgut	Present	Regionally restricted	5B	Expression excluded most of hindgut endoderm.
TS14: embryo mesoderm	Present	Scattered	5B	Scattered expressing cells in mesoderm spanning notocord and expressing cells in tailgut mesoderm.
TS14: allantois	Present	Widespread	5B	Expressing throughout allantois.

Structure	Level	Pattern	Image	Note
TS14: embryo mesoderm	Present	Regionally restricted	5C	Punctate expressing cells between tailbud mesoderm and notochord populations.
TS14: primitive hindgut	Present	Regionally restricted	5C	Expression interspersed within dorsal hindgut endoderm.
TS14: surface ectoderm	Present	Single cells	5C	Occasional expressing cell in embryonic surface ectoderm.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from splanchnopleure	Present	Regionally restricted	5D	Expression in posterior splanchnopleure as it folds towards midline.
TS14: notochord	Present		5D
TS14: yolk sac	Present	Regionally restricted	5D	Expression in visceral yolk sac.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from splanchnopleure	Present	Regionally restricted	5E	Expression in posterior splanchnopleure as it folds towards midline.
TS14: primitive hindgut	Present	Regionally restricted	5E	Expression in hindgut endoderm immediately posterior to midline joining of splanchnopleure.
TS14: notochord	Present		5E

Structure	Level	Pattern	Image	Note
TS14: vitelline blood vessel	Present	Regionally restricted	5F	Expression as vitelline vessels join at midline to form omphalomesenteric artery.
TS14: notochord	Present		5F

Structure	Level	Pattern	Image	Note
TS14: primitive hindgut	Ambiguous	Regionally restricted	5G	Posterior to fusion of vitelline vessels, expression wanes within hindgut and omphalomesenteric artery. Authors state expression fades to undetectable.
TS14: vitelline artery	Ambiguous	Regionally restricted	5G	Posterior to fusion of vitelline vessels, expression wanes within hindgut and omphalomesenteric artery. Authors state expression fades to undetectable.

Structure	Level	Pattern	Image	Note
TS14: notochord	Present	Punctate	5H	Limited punctate expressing cells in notochord.
TS14: yolk sac endoderm	Weak		5H	Relatively weak intensity of expression in visceral yolk sac endoderm.

Structure	Level	Pattern	Image	Note
TS11: Allantoic Core Domain	Strong		6A	Greatest intensity of expression within Allantoic core Domain.

Structure	Level	Pattern	Image	Note
TS11: allantois	Weak	Regionally restricted	6B	Faint expression throughout distal allantois and mesothelium.

Structure	Level	Pattern	Image	Note
TS11: Allantoic Core Domain	Strong	Widespread	6C	Intense expression throughout Allantoic core Domain.
TS11: allantois-associated posterior visceral endoderm	Strong	Regionally restricted	6C	Intense expression within allantois-associated posterior visceral endoderm near site of presumptive vessel of confluence.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Regionally restricted	6D	Expression co-localizing with Runx1 within proximal allantois.
TS12: posterior crown of node	Present	Regionally restricted	6D	Expression within presumptive posterior crown population.

Structure	Level	Pattern	Image	Note
TS12: umbilical blood vessel	Strong	Regionally restricted	6E	In nascent umbilical vasculature in proximal allantois, strong expression in more proximal cells.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Regionally restricted		Co-expression with Runx1 in ventral allantoic mesothelium.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Strong	Regionally restricted	6F	Expression most intensely localized to primitive streak-associated cells adjacent to hindgut invagination.
TS12: embryo mesoderm	Weak	Regionally restricted	6F	Faint colocalization with Runx1 reporter expression within ventral hindgut lip and proximo-ventral mesoderm.
TS12: primitive hindgut	Weak	Regionally restricted	6F	Faint colocalization with Runx1 reporter expression within ventral hindgut lip and proximo-ventral mesoderm.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Strong	Regionally restricted	6G	Expression most intensely localized to primitive streak-associated cells adjacent to hindgut invagination.
TS12: embryo mesoderm	Weak	Regionally restricted	6G	Faint colocalization with Runx1 reporter expression within ventral hindgut lip and proximo-ventral mesoderm.
TS12: primitive hindgut	Weak	Regionally restricted	6G	Faint colocalization with Runx1 reporter expression within ventral hindgut lip and proximo-ventral mesoderm.

Structure	Level	Pattern	Image	Note
TS13: primitive hindgut	Present	Regionally restricted	6H	Expression in hindgut lip. Expression excluded from hindgut endoderm more posterior to hindgut lip.
TS13: blood island	Present		6H
TS13: vitelline artery	Present	Regionally restricted	6H	Expression localized to developing blood cells of the omphalomesenteric artery.

Structure	Level	Pattern	Image	Note
TS13: yolk sac mesenchyme	Present	Widespread	6I	Expression throughout yolk sac/blood island mesoderm.
TS13: extraembryonic endoderm	Strong	Regionally restricted	6I	Expression strongly colocalizing with Runx1 reporter expression in extraembryonic visceral endoderm.
TS13: yolk sac	Present	Regionally restricted	6I	Within visceral yolk sac, co-localization with Runx1 in both endothelium and blood cells. Stronger expression in endothelium.
TS13: blood island	Present	Regionally restricted	6I	Expression colocalizes with Runx1 reporter expression in presumptive erythroblasts.

Structure	Level	Pattern	Image	Note
TS13: chorion ectoderm	Present	Regionally restricted	6J	Expression in lateral chorionic ectoderm adjacent to site of contiguity with yolk sac. Co-expression to a lesser extent within lateral chorionic ectoderm.
TS13: extraembryonic endoderm	Present	Regionally restricted	6J	Colocalization with Runx1 reporter expression within extraembryonic visceral endoderm contiguous with yolk sac.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Absent		6K	No expression in neural tube or brain.
TS13: amnion	Present	Widespread	6K	Expression throughout amnion and embryonic surface ectodderm.
TS13: surface ectoderm	Present	Widespread	6K	Expression throughout amnion and embryonic surface ectodderm.
TS13: blood island	Present	Regionally restricted	6K	Expression in yolk sac, blood islands and primitive erythroblasts.
TS13: yolk sac	Present	Regionally restricted	6K	Expression in yolk sac, blood islands and primitive erythroblasts.

Structure	Level	Pattern	Image	Note
TS13: blood island	Present	Regionally restricted	6K inset	Expression in some but not all circulating primitive erythroblasts within embryonic vasculature.

Structure	Level	Pattern	Image	Note
TS13: dorsal aorta	Absent		6L	Authors report no expression within any of the structures indicated in the figure.
TS13: foregut diverticulum	Absent		6L	Authors report no expression within any of the structures indicated in the figure.
TS13: heart	Absent		6L	Authors report no expression within any of the structures indicated in the figure.
TS13: neural tube	Absent		6L	Authors report no expression within any of the structures indicated in the figure.
TS13: notochord	Absent		6L	Authors report no expression within any of the structures indicated in the figure.
TS13: paraxial mesenchyme	Absent		6L	Authors report no expression within any of the structures indicated in the figure.