Arl13b Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:287091 Gigante ED, et al., ARL13B regulates Sonic hedgehog signaling from outside primary cilia. Elife. 2020 Mar 4;9:e50434
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6436860
Gene symbol:	Arl13b
Gene name:	ADP-ribosylation factor-like 13B

Antibody:	anti-ARL13B [N295B/66] ab136648
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Results

2C +/+ ©

Specimen 2C +/+: embryonic day 10.5 (more

)
Note: Double labeled: magenta - Arl13b; green - Ift88 (assay MGI:6436857)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2C +/+ magenta , 2C +/+	Expression is seen in the cilia.

2C V358A/+ ©

Specimen 2C V358A/+: embryonic day 10.5; Arl13b^em1Tc/Arl13b⁺ (more

)
Note: Double labeled: magenta - Arl13b; green - Ift88 (assay MGI:6436857)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2C V358A/+ , 2C V358A/+ magenta	Expression is seen in the cilia.

Specimen 2C V358A/V358A: embryonic day 10.5; Arl13b^em1Tc/Arl13b^em1Tc (more

)
Note: Double labeled: magenta - Arl13b; green - Ift88 (assay MGI:6436857)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Absent		2C V358A/V358A magenta , 2C V358A/V358A	No expression is seen in the cilia.

Specimen 2 S1 +/+: embryonic day 10.5 (more

)
Note: Wild type littermate. Double labeled: magenta - Arl13b; green - Ift88 (assay MGI:6436857); overexposed image

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2 S1_+/+ magenta	Expression is seen in the cilia.

Specimen 2 S1 A/A: embryonic day 10.5; Arl13b^em1Tc/Arl13b^em1Tc (more

)
Note: Double labeled: magenta - Arl13b; green - Ift88 (assay MGI:6436857); overexposed image

Structure	Level	Pattern	Image	Note
TS17: neural tube	Weak	Regionally restricted	2 S1_A/A magenta	Low level expression is seen throughout the cell; no expression is seen in the cilia.

Specimen 2 S1 hnn/hnn: embryonic day 10.5; Arl13b^hnn/Arl13b^hnn (more

)
Note: Double labeled: magenta - Arl13b; green - Ift88 (assay MGI:6436857); overexposed image

Structure	Level	Pattern	Image	Note
TS17: neural tube	Absent		2 S1_hnn/hnn magenta

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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