Ift88 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:287091 Gigante ED, et al., ARL13B regulates Sonic hedgehog signaling from outside primary cilia. Elife. 2020 Mar 4;9:e50434
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6436857
Gene symbol:	Ift88
Gene name:	intraflagellar transport 88

Antibody:	anti-Ift88
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

2C +/+ ©

2C +/+ green ©

Specimen 2C +/+: embryonic day 10.5 (more

)
Note: Double labeled: green - Ift88; magenta - Arl13b (assay MGI:6436860)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2C +/+ , 2C +/+ green	Expression is seen in the cilia.

2C V358A/+ ©

Specimen 2C V358A/+: embryonic day 10.5; Arl13b^em1Tc/Arl13b⁺ (more

)
Note: Double labeled: green - Ift88; magenta - Arl13b (assay MGI:6436860)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2C V358A/+ green , 2C V358A/+	Expression is seen in the cilia.

Specimen 2C V358A/V358A: embryonic day 10.5; Arl13b^em1Tc/Arl13b^em1Tc (more

)
Note: Double labeled: green - Ift88; magenta - Arl13b (assay MGI:6436860)

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2C V358A/V358A , 2C V358A/V358A green	Expression is seen in the cilia.

Specimen 2 S1 +/+: embryonic day 10.5 (more

)
Note: Wild type littermate. Double labeled: green - Ift88; magenta - Arl13b (assay MGI:6436860); overexposed image

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2 S1_+/+ green	Expression is seen in the cilia.

Specimen 2 S1 A/A: embryonic day 10.5; Arl13b^em1Tc/Arl13b^em1Tc (more

)
Note: Double labeled: green - Ift88; magenta - Arl13b (assay MGI:6436860); overexposed image

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2 S1_A/A green	Expression is seen in the cilia.

Specimen 2 S1 hnn/hnn: embryonic day 10.5; Arl13b^hnn/Arl13b^hnn (more

)
Note: Double labeled: green - Ift88; magenta - Arl13b (assay MGI:6436860); overexposed image

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2 S1_hnn/hnn green	Expression is seen in the cilia.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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