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Gene Expression Data
Assay Details
Assay
Reference: J:286601 Jiang Y, et al., CXXC finger protein 1-mediated histone H3 lysine-4 trimethylation is essential for proper meiotic crossover formation in mice. Development. 2020 Mar 17;147(6):dev183764
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6435189
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phospho-histone H2A.X (20E3) (CST;#9718)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: The tissue was fixed in either Bouin's or 4% paraformaldehyde.
Results
Specimen 2D - WT: postnatal week 12 (more )
Note: Wild-type littermate control; it retains at least one floxed allele.
Structure Level Pattern Image Note
TS28: germ cell of testis Present 2D WT gH2AX Double strand breaks are stained.
Specimen 2D - mut: postnatal week 12; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: germ cell of testis Present 2D mut gH2AX Double strand breaks are stained.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory