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Gene Expression Data
Assay Details
Assay
Reference: J:285969 Liu H, et al., SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice. Nucleic Acids Res. 2019 Jun 20;47(11):5670-5683
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6431464
Gene symbol: Mlh1
Gene name: mutL homolog 1
Antibody: Anti-Mlh1 (G168-15)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4Z wild type: postnatal (more )
Note: Double labeled: green - Mlh1; red - Sycp3 (assay MGI:6431459).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4Z upper Expression is present at sites of crossover in the mid-late pachytene spermatocytes.
Specimen 4Z mutant: postnatal; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: green - Mlh1; red - Sycp3 (assay MGI:6431459).
Structure Level Pattern Image Note
TS28: spermatocyte Absent 4Z lower In wild type, expression is present at sites of crossover in the mid-late pachytene spermatocytes; since the mutants do not progress to this stage, there is no expression.
Specimen S6d: embryonic day 17.5 (more )
Note: Wild type. Double labeled: green - Mlh1; red - Sycp3 (assay MGI:6431459).
Structure Level Pattern Image Note
TS25: oocyte Present S6d Expression is present at sites of crossover in the pachytene oocytes.
Specimen S6e: embryonic day 17.5; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: green - Mlh1; red - Sycp3 (assay MGI:6431459).
Structure Level Pattern Image Note
TS25: oocyte Absent S6e In wild type, expression is present at sites of crossover in the pachytene oocytes; since the mutants do not progress to this stage, there is no expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory