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Gene Expression Data
Assay Details
Assay
Reference: J:173839 Bertrand N, et al., Hox genes define distinct progenitor sub-domains within the second heart field. Dev Biol. 2011 May 15;353(2):266-74
Assay type: RNA in situ
MGI Accession ID: MGI:6430416
Gene symbol: Hoxb1
Gene name: homeobox B1
Probe: Hoxb1 probe29
Probe preparation: Antisense labelled with fluorescein RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1B: embryonic day 7.75 (more )
Note: Double labeled: orange - Hoxb1; blue - Tbx5 (assay MGI:6430433).
Structure Level Pattern Image Note
TS12: embryo mesoderm Present Regionally restricted 1B Anterior border of expression does not overlap with Tbx5, indicating Hoxb1 is not expressed in cardiac crescent.
Specimen 1C: embryonic day 7.75 (more )
Note: Double labeled: orange - Hoxb1; blue - Isl1 (assay MGI:6430434).
Structure Level Pattern Image Note
TS12: cardiogenic plate Present Regionally restricted 1C
TS12: secondary heart field Present Regionally restricted 1C Expression in posterior domain of second heart field.
TS12: endocardial tube Absent 1C No expression in heart tube.
Specimen S2A: embryonic day 8.5 (more )
Note: Double labeled: orange - Hoxb1; blue - Isl1 (assay MGI:6430434).
Structure Level Pattern Image Note
TS13: secondary heart field Present S2A
TS13: endocardial tube Absent S2A No expression in heart tube.
Specimen S2A': embryonic day 8.5 (more )
Note: Double labeled: orange - Hoxb1; blue - Isl1 (assay MGI:6430434).
Structure Level Pattern Image Note
TS13: foregut diverticulum endoderm Present Regionally restricted S2A' Expression anterior foregut endoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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