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Gene Expression Data
Assay Details
Assay
Reference: J:261117 Sasamoto K, et al., Cdk5 is required for the positioning and survival of GABAergic neurons in developing mouse striatum. Dev Neurobiol. 2017 Apr;77(4):483-492
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6429943
Gene symbol: Ppp1r1b
Gene name: protein phosphatase 1, regulatory inhibitor subunit 1B
Antibody: anti-DARPP-32
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 1B/B' +/+: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: ventral striatum Present 1B +/+ , 1B' +/+ Expressed in GABAergic neurons.
Specimen 1B/B' -/-: embryonic day 18.5; Cdk5tm1Kul/Cdk5tm1Kul (more )
Structure Level Pattern Image Note
TS26: ventral striatum Present Regionally restricted 1B -/- , 1B' -/- Expressed in GABAergic neurons. The distribution was altered in the mutant. The staining revealed the absence of structure of the olfactory tubercles.
Specimen 3A control: postnatal day 1 (more )
Note: Littermate control, but genotype was not specified.
Structure Level Pattern Image Note
TS27: accumbens nucleus Present 3A left
TS27: olfactory tubercle Present 3A left
Specimen 3A Dlx5/6-p35cKO; p39KO: postnatal day 1; Cdk5r1tm2Ohsh/Cdk5r1tm2Ohsh, Cdk5r2tm1Lht/Cdk5r2tm1Lht, Tg(dlx5a-cre)1Mekk/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: accumbens nucleus Absent 3A right
TS27: olfactory tubercle Absent 3A right
Specimen 3C Control: postnatal day 14 (more )
Note: Littermate control, but genotype was not specified.
Structure Level Pattern Image Note
TS28: accumbens nucleus Present 3C upper left
TS28: olfactory tubercle Present 3C upper left
Specimen 3C Dlx5/6-p35cKO; p39KO: postnatal day 14; Cdk5r1tm2Ohsh/Cdk5r1tm2Ohsh, Cdk5r2tm1Lht/Cdk5r2tm1Lht, Tg(dlx5a-cre)1Mekk/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: accumbens nucleus Absent 3C upper right
TS28: olfactory tubercle Absent 3C upper right
Specimen 3D upper control: postnatal day 21 (more )
Note: Littermate control, but genotype was not specified.
Structure Level Pattern Image Note
TS28: accumbens nucleus Present 3D upper left
TS28: olfactory tubercle Present 3D upper left
TS28: ventral striatum Present 3D upper left
Specimen 3D upper Dlx5/6-p35cKO; p39KO: postnatal day 21; Cdk5r1tm2Ohsh/Cdk5r1tm2Ohsh, Cdk5r2tm1Lht/Cdk5r2tm1Lht, Tg(dlx5a-cre)1Mekk/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: accumbens nucleus Absent 3D upper right
TS28: olfactory tubercle Absent 3D upper right
TS28: ventral striatum Present Regionally restricted 3D upper right Fewer GABAergic neurons compared with control.
Specimen 3D lower control: postnatal day 21 (more )
Note: Littermate control, but genotype was not specified.
Structure Level Pattern Image Note
TS28: accumbens nucleus Present 3D lower left
TS28: olfactory tubercle Present 3D lower left
TS28: ventral striatum Present 3D lower left
Specimen 3D lower Dlx5/6-p35cKO; p39KO: postnatal day 21; Cdk5r1tm2Ohsh/Cdk5r1tm2Ohsh, Cdk5r2tm1Lht/Cdk5r2tm1Lht, Tg(dlx5a-cre)1Mekk/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: accumbens nucleus Absent 3D lower right
TS28: olfactory tubercle Absent 3D lower right
TS28: ventral striatum Present Regionally restricted 3D lower right Fewer GABAergic neurons compared with control.
Specimen not shown: postnatal (more )
Structure Level Pattern Image Note
TS28: accumbens nucleus Present
TS28: olfactory tubercle Present
Specimen not shown: postnatal; Cdk5r1tm2Ohsh/Cdk5r1tm2Ohsh, Tg(dlx5a-cre)1Mekk/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: accumbens nucleus Present Same as in the control.
TS28: olfactory tubercle Present Same as in the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory