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Gene Expression Data
Assay Details
Assay
Reference: J:272566 Yang R, et al., CDK5RAP3, a UFL1 substrate adaptor, is crucial for liver development. Development. 2019 Jan 25;146(2):dev169235
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6423287
Gene symbol: Cdk5rap3
Gene name: CDK5 regulatory subunit associated protein 3
Antibody: Anti-CDK5RAP3 (H-300; sc-134627)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Two antibodies were described MGI:6423118 and MGI:6423120, but the one used in this assay was not specified. Either antibody could have been used.
Results
Specimen 2A: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: liver Present Regionally restricted 2A Expression was detected in hepatocytes.
Specimen 2A': embryonic day 16.5; Cdk5rap3tm1b(EUCOMM)Hmgu/Cdk5rap3tm1b(EUCOMM)Hmgu (more )
Structure Level Pattern Image Note
TS24: liver Ambiguous 2A'
Specimen 4D: postnatal month 1 (more )
Note: The conditional control specimen was a littermate.
Structure Level Pattern Image Note
TS28: liver Present 4D
Specimen 4D': postnatal month 1; Cdk5rap3tm1c(EUCOMM)Hmgu/Cdk5rap3tm1c(EUCOMM)Hmgu, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: liver Ambiguous 4D' The conditional knockout had a relatively high knockout efficiency.
Specimen S4F Con: postnatal month 3 (more )
Note: The conditional control specimen was a littermate.
Structure Level Pattern Image Note
TS28: liver Present S4F Con
Specimen S4F tm1d/tm1d;Alb-Cre: postnatal month 3; Cdk5rap3tm1c(EUCOMM)Hmgu/Cdk5rap3tm1c(EUCOMM)Hmgu, Speer6-ps1Tg(Alb-cre)21Mgn/Speer6-ps1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: liver Present Regionally restricted S4F tm1d/tm1d;Alb-Cre There was insufficient deletion of Cdk5rap3 in hepatocytes of the tm1d/tm1d;Alb-Cre liver.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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