About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:166736 Tee WW, et al., Prmt5 is essential for early mouse development and acts in the cytoplasm to maintain ES cell pluripotency. Genes Dev. 2010 Dec 15;24(24):2772-7
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6415679
Gene symbol: Prmt5
Gene name: protein arginine N-methyltransferase 5
Antibody: anti-Prmt5 (07-405)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A Oocyte: postnatal adult (more )
Structure Level Pattern Image Note
TS28: oocyte Present 1A Oocyte Merge , 1A Prmt5 Oocyte Protein is in the cytoplasm.
Specimen 1A Zygote: embryonic day 0.5 (more )
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 1A Prmt5 Zygote , 1A Zygote Merge Protein is in the cytoplasm.
Specimen 1A 8-cell: embryonic day 2.5 (more )
Note: 8-cell stage embryo. Double labeled: red - Prmt5; green - Pou5f1 (assay MGI:6415680).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 1A Prmt5 8-cell , 1A 8-cell Merge
Specimen 1A Morula: embryonic day 3.0 (more )
Note: Morula stage embryo. Double labeled: red - Prmt5; green - Pou5f1 (assay MGI:6415680).
Structure Level Pattern Image Note
TS3: compacted morula Present 1A Morula Merge , 1A Prmt5 Morula
Specimen 1A E3.5: embryonic day 3.5 (more )
Note: Early blastocyst stage embryo. Double labeled: red - Prmt5; green - Pou5f1 (assay MGI:6415680).
Structure Level Pattern Image Note
TS4: inner cell mass Present 1A Prmt5 E3.5 , 1A E3.5 Merge Protein is nuclear.
TS4: trophectoderm Present 1A Prmt5 E3.5 , 1A E3.5 Merge Protein is nuclear.
Specimen 1A E4.5: embryonic day 4.5 (more )
Note: Double labeled: red - Prmt5; green - Pou5f1 (assay MGI:6415680).
Structure Level Pattern Image Note
TS6: trophectoderm Present 1A Prmt5 E4.5 , 1A E4.5 Merge Protein is nuclear.
TS6: epiblast Weak 1A E4.5 Merge , 1A Prmt5 E4.5
Specimen 1A E6.5: embryonic day 6.5 (more )
Note: Double labeled: red - Prmt5; green - Prdm1 (assay MGI:6416075).
Structure Level Pattern Image Note
TS9: embryo Present 1A Prmt5 E6.5 , 1A E6.5 Merge Protein is predominantly cytoplasmic and is upregulated in the epiblast.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory