Probe:	Ablim1 3'UTRa
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted	1h	Asymmetric expression.
TS13: node	Present	Regionally restricted	1h	Asymmetric and weaker expression to the right side.
TS13: head	Present		1h	Symmetric expression.
TS13: heart	Present		1h	Symmetric expression.

Structure	Level	Pattern	Image	Note
TS13: head	Present		1l	Symmetric expression.
TS13: node	Present	Regionally restricted	1l	Asymmetric and weaker expression to the right side.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present		2a	Bilaterally symmetrical expression.
TS12: heart	Present		2a

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	2b	Asymmetric expression, with expression in the left being both stronger and stretching further posteriorly than in the right.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	2c	Asymmetric expression, with expression in the left being both stronger and stretching further posteriorly than in the right.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	2d	Asymmetric left lateral plate expression was strongly downregulated, although still evident at a low level in some embryos.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present		2e	No asymmetric expression.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present		2f , 2g	Expression was present throughout the lateral plate mesoderm.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	3a	Expressed on the left side in the mutant (11%, see Table 1).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	3b	Bilateral expression in the mutant (44%, see Table 1).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	3c	Expressed on the right side in the mutant (28%, see Table 1).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	3d	Asymmetric expression in left side (56%, see Table 1).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	3e	Expressed on the left side in the mutant, same as in the wild-type (100%, see Table 1).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent		3f	No expression in the mutant (76%, see Table 1).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	3g	Expressed on the left side in the mutant (24%, see Table 1).

Structure	Level	Pattern	Image	Note
TS11: lateral plate mesoderm	Present			Bilaterally symmetrical expression.
TS11: heart	Present

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present			Bilaterally symmetrical expression (100%, see Table 1).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			No expression in the mutant (17%, see Table 1).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			No expression in the mutant (44%, higher than in the wild-type (20%), see Table 1).