Antibody:	anti-MacroD2
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Strong		4Ab , 4Aa	Expression is seen in neurons.
TS27: cortical layer III	Strong		4Ab , 4Aa	Expression is seen in neurons.
TS27: cortical layer IV	Strong		4Ab , 4Aa	Expression is seen in neurons.
TS27: cortical layer V	Strong		4Ac , 4Aa	Expression is seen in neurons.
TS27: cortical layer VI	Weak		4Aa	Expression is seen in neurons.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Strong		4Bb , 4Ba	Expression is seen in most neurons.
TS28: cortical layer III	Strong		4Bb , 4Ba	Expression is seen in most neurons.
TS28: cortical layer IV	Strong		4Bb , 4Ba	Expression is seen in most neurons.
TS28: cortical layer V	Present	Regionally restricted	4Bc , 4Ba	Expression is seen in a limited of number of neurons.
TS28: cortical layer VI	Weak		4Ba	The majority of neurons show low signals.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Weak	Non-Uniform	4Cb , 4Ca	Neuronal staining is weak and sparse.
TS28: cortical layer III	Weak	Non-Uniform	4Cb , 4Ca	Neuronal staining is weak and sparse.
TS28: cortical layer IV	Weak	Non-Uniform	4Cb , 4Ca	Neuronal staining is weak and sparse.
TS28: cortical layer V	Weak	Non-Uniform	4Cc , 4Ca	Neuronal staining is weak and sparse.
TS28: cortical layer VI	Weak	Non-Uniform	4Ca	Neuronal staining is weak and sparse.

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA1	Present		5Ac , 5Aa , 5Ab	Expression is observed in the soma of pyramidal cells.
TS27: hippocampus CA2	Present		5Ac , 5Aa	Expression is observed in the soma of pyramidal cells.
TS27: hippocampus CA3	Present		5Ad , 5Aa	Expression is observed in the soma of pyramidal cells.
TS27: dentate gyrus granule cell layer	Present		5Ae , 5Aa	Expression is observed in the soma.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present		5Bb , 5Bc , 5Ba	Expression is observed in the soma of pyramidal cells.
TS28: hippocampus CA2	Present		5Bc , 5Ba	Expression is observed in the soma of pyramidal cells.
TS28: hippocampus CA3	Present		5Bd , 5Ba	Expression is observed in the soma of pyramidal cells, although expression levels have decreased in this region.
TS28: dentate gyrus granule cell layer	Present		5Ba , 5Be	Expression is observed in the soma.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Widespread	5Ca	Expression is observed widely throughout the hippocampal regions and layers.
TS28: hippocampus CA1	Present	Widespread	5Ca , 5Cc , 5Cb
TS28: hippocampus CA2	Present	Widespread	5Cc , 5Ca
TS28: hippocampus CA3	Present	Widespread	5Ca , 5Cd
TS28: dentate gyrus granule cell layer	Weak	Widespread	5Ca , 5Ce	Expression is seen in the majority of granular cells.
TS28: dentate gyrus	Strong	Single cells	5Ca , 5Ce	A limited number of unidentified small cells exhibited strong staining.