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Gene Expression Data
Assay Details
Assay
Reference: J:224632 Lowe KL, et al., Podoplanin and CLEC-2 drive cerebrovascular patterning and integrity during development. Blood. 2015 Jun 11;125(24):3769-77
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6407096
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-PECAM-1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2A +/+: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: brain blood vessel Present 2A +/+ left , 2A +/+ right , 2A +/+ middle Organized, characteristic branching patterns.
Specimen 2A Clec-2-/-: embryonic day 10.5; Clec1btm1.2Arte/Clec1btm1.2Arte (more )
Structure Level Pattern Image Note
TS17: brain blood vessel Present 2A Clec-2-/- right , 2A Clec-2-/- left , 2A Clec-2-/- middle Vasculature appeared tortuous and abnormally patterned, compared with the organized, characteristic branching patterns of littermates.
Specimen 2A Pdpn PGK-cKO: embryonic day 10.5; Pdpntm1Arte/Pdpntm1Arte, Tg(Pgk1-cre)1Lni/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: brain blood vessel Present 2A Pdpn cKO left , 2A Pdpn cKO right , 2A Pdpn cKO middle Vasculature appeared tortuous and abnormally patterned, compared with the organized, characteristic branching patterns of littermates.
Specimen 2B +/+: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: brain blood vessel Present 2B +/+ middle , 2B +/+ right , 2B +/+ left Organized, characteristic branching patterns.
Specimen 2B Clec-2-/-: embryonic day 12.5; Clec1btm1.2Arte/Clec1btm1.2Arte (more )
Structure Level Pattern Image Note
TS20: brain blood vessel Present 2B Clec-2-/- middle , 2B Clec-2-/- left , 2B Clec-2-/- right Hemorrhages were clearly visible throughout the developing fore-, mid-, and hindbrain, as indicated by dense areas staining. Hemorrhages formed among aberrantly patterned vascular networks.
Specimen 2B Pdpn PGK-cKO: embryonic day 12.5; Pdpntm1Arte/Pdpntm1Arte, Tg(Pgk1-cre)1Lni/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: brain blood vessel Present 2B Pdpn cKO left , 2B Pdpn cKO right , 2B Pdpn cKO middle Hemorrhages were clearly visible throughout the developing fore-, mid-, and hindbrain, as indicated by dense areas staining. Hemorrhages formed among aberrantly patterned vascular networks.
Specimen 4A +/+: embryonic day 11.5 (more )
Note: Triple labeled: green - Pecam1; red - Pdpn (assay MGI:6407082); blue - Cspg4 (assay MGI:6407113).
Structure Level Pattern Image Note
TS19: brain blood vessel Present 4A +/+ merge , 4A +/+ Pecam-1
Specimen 4A Clec-2-/-: embryonic day 11.5; Clec1btm1.2Arte/Clec1btm1.2Arte (more )
Note: Triple labeled: green - Pecam1; red - Pdpn (assay MGI:6407082); blue - Cspg4 (assay MGI:6407113).
Structure Level Pattern Image Note
TS19: brain blood vessel Present 4A Clec-2-/- merge , 4A Clec-2-/- Pecam-1
Specimen 4A Pdpn PGK-cKO: embryonic day 11.5; Pdpntm1Arte/Pdpntm1Arte, Tg(Pgk1-cre)1Lni/0 (more )
Note: Conditional mutant. Triple labeled: green - Pecam1; red - Pdpn (assay MGI:6407082); blue - Cspg4 (assay MGI:6407113).
Structure Level Pattern Image Note
TS19: brain blood vessel Present 4A Pdpn PGK cKO merge , 4A Pdpn PGK cKO Pecam-1
Specimen 4A Pdpn Nes-cKO: embryonic day 11.5; Pdpntm1Arte/Pdpntm1Arte, Tg(Nes-cre)1Wmz/0 (more )
Note: Conditional mutant. Triple labeled: green - Pecam1; red - Pdpn (assay MGI:6407082); blue - Cspg4 (assay MGI:6407113).
Structure Level Pattern Image Note
TS19: brain blood vessel Present 4A Pdpn Nes cKO merge , 4A Pdpn Nes cKO Pecam-1
Specimen S3 +/+: embryonic day 14.5 (more )
Note: Triple labeled: green - Pecam1; blue - Ly76 (assay MGI:6407115).; red - Itga2b (assay MGI:6407116).
Structure Level Pattern Image Note
TS22: brain blood vessel Present S3_top middle , S3_top right , S3_top left
Specimen S3 Clec-2-/-: embryonic day 14.5; Clec1btm1.2Arte/Clec1btm1.2Arte (more )
Note: Triple labeled: green - Pecam1; blue - Ly76 (assay MGI:6407115).; red - Itga2b (assay MGI:6407116).
Structure Level Pattern Image Note
TS22: choroid plexus Present S3_bottom middle , S3_bottom left
TS22: brain blood vessel Present S3_bottom left , S3_bottom right
Specimen S5: embryonic day 12.5; Pdpntm1Arte/Pdpntm1Arte, Tg(Nes-cre)1Wmz/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: brain blood vessel Present S5_middle , S5_right , S5_left Smaller hemorrhages were clearly visible throughout the developing fore-, mid-, and hindbrain, as indicated by dense areas staining. Hemorrhages formed among visibly tortuous blood vessels.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory