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Gene Expression Data
Assay Details
Assay
Reference: J:243205 Havrilak JA, et al., Endothelial cells are not required for specification of respiratory progenitors. Dev Biol. 2017 Jul 01;427(1):93-105
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6404848
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Results
Specimen 1A: embryonic day 8.5 (more )
Note: 10 somites. Double labeled: green - Nkx2-1; red - Foxa2 (assay MGI:6404849).
Structure Level Pattern Image Note
TS13: future prosencephalon Present 1A Expression in forebrain.
TS13: thyroid primordium Present 1A Expression in thyroid region.
TS13: foregut diverticulum Present Regionally restricted 1A No expression in presumptive respiratory field in ventral foregut endoderm dorsal to developing heart.
Specimen 1B: embryonic day 9.0 (more )
Note: 14 somites. Double labeled: green - Nkx2-1; red - Foxa2 (assay MGI:6404849).
Structure Level Pattern Image Note
TS14: prosencephalon Present 1B Expression in forebrain.
TS14: thyroid primordium Present 1B Expression in thyroid region.
TS14: foregut diverticulum Present Regionally restricted 1B No expression in presumptive respiratory field in ventral foregut endoderm dorsal to developing heart.
Specimen 1C: embryonic day 9.0 (more )
Note: 16 somites. Double labeled: green - Nkx2-1; red - Foxa2 (assay MGI:6404849).
Structure Level Pattern Image Note
TS14: prosencephalon Present 1C Expression in forebrain.
TS14: thyroid primordium Present 1C Expression in thyroid region.
TS14: foregut Present Regionally restricted 1C Expression cells in presumptive lung field in ventral foregut endoderm dorsal to developing heart.
Specimen 1D: embryonic day 9.0 (more )
Note: 16 somites. Triple labeled: green - Nkx2-1; red - Emcn (assay MGI:6404850); white - Cdh1 (assay MGI:6404851).
Structure Level Pattern Image Note
TS14: foregut Present Regionally restricted 1D Expression in respiratory progenitors emerging from ventral side of foregut in proximity to endothelial cells.
Specimen 3C: embryonic day 9.5 (more )
Note: Double labeled: green - Nkx2-1; red - Pecam1 (assay MGI:6404852).
Structure Level Pattern Image Note
TS15: thyroid primordium Present 3C Expression in thyroid region.
TS15: lung Present 3C Expression in prospective lung field.
Specimen 3D: embryonic day 9.5; Kdrorv/Kdrorv (more )
Note: Double labeled: green - Nkx2-1; red - Pecam1 (assay MGI:6404852).
Structure Level Pattern Image Note
TS15: thyroid primordium Present 3D Expression in thyroid region.
TS15: lung Ambiguous 3D Expression in region where respiratory progenitors are found limited to a small number of cells in 81% (17/21) of embryos examined.
Specimen 4B: embryonic day 8.5 (more )
Note: Triple labeled: green - Nkx2-1; red - Emcn (assay MGI:6404850); white - Cdh1 (assay MGI:6404851).
Structure Level Pattern Image Note
TS13: future prosencephalon Present 4B Expression in forebrain.
Specimen 4E: embryonic day 8.5; Kdrorv/Kdrorv (more )
Note: Triple labeled: green - Nkx2-1; red - Emcn (assay MGI:6404850); white - Cdh1 (assay MGI:6404851).
Structure Level Pattern Image Note
TS13: future prosencephalon Present 4E Expression in forebrain.
Specimen 6A: embryonic day 8.5 (more )
Note: Triple labeled: green - Nkx2-1; red - Pecam1 (assay MGI:6404852); white - Hnf4a (assay MGI:6404853).
Structure Level Pattern Image Note
TS13: thyroid primordium Present 6A
Specimen 6B: embryonic day 8.5; Kdrorv/Kdrorv (more )
Note: Triple labeled: green - Nkx2-1; red - Pecam1 (assay MGI:6404852); white - Hnf4a (assay MGI:6404853).
Structure Level Pattern Image Note
TS13: thyroid primordium Present 6B
Specimen 6C: embryonic day 9.5 (more )
Note: Triple labeled: green - Nkx2-1; red - Pecam1 (assay MGI:6404852); white - Pdx1 (assay MGI:6404854).
Structure Level Pattern Image Note
TS15: thyroid primordium Present 6C
TS15: lung Present 6C Expression in respiratory progenitors.
Specimen 6D: embryonic day 9.5; Kdrorv/Kdrorv (more )
Note: Triple labeled: green - Nkx2-1; red - Pecam1 (assay MGI:6404852); white - Pdx1 (assay MGI:6404854).
Structure Level Pattern Image Note
TS15: thyroid primordium Present 6D
TS15: lung Ambiguous 6D Expression in region where respiratory progenitors are found limited to a small number of cells in 81% (17/21) of embryos examined.
Specimen 6E: embryonic day 9.5 (more )
Note: Triple labeled: green - Nkx2-1; red - Pecam1 (assay MGI:6404852); white - Hnf4a (assay MGI:6404853).
Structure Level Pattern Image Note
TS15: thyroid primordium Present 6E
TS15: lung Present 6E Expression in respiratory progenitors.
Specimen 6F: embryonic day 9.5; Kdrorv/Kdrorv (more )
Note: Triple labeled: green - Nkx2-1; red - Pecam1 (assay MGI:6404852); white - Hnf4a (assay MGI:6404853).
Structure Level Pattern Image Note
TS15: thyroid primordium Present 6F
TS15: lung Ambiguous 6F Expression in region where respiratory progenitors are found limited to a small number of cells in 81% (17/21) of embryos examined.
Specimen 6G: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-1; red - Pecam1 (assay MGI:6404852); white - Pdx1 (assay MGI:6404854).
Structure Level Pattern Image Note
TS17: thyroid primordium Present 6G
TS17: lung Present 6G Expression in respiratory progenitors.
Specimen 6H: embryonic day 10.5; Kdrorv/Kdrorv (more )
Note: Triple labeled: green - Nkx2-1; red - Pecam1 (assay MGI:6404852); white - Pdx1 (assay MGI:6404854).
Structure Level Pattern Image Note
TS17: thyroid primordium Present 6H
TS17: lung Absent 6H No respiratory expression seen in E10.5 mutant embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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