About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:269500 Hao MM, et al., Arundic Acid Prevents Developmental Upregulation of S100B Expression and Inhibits Enteric Glial Development. Front Cell Neurosci. 2017;11:42
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6403737
Gene symbol: S100b
Gene name: S100 protein, beta polypeptide, neural
Antibody: anti-S-100 (Z0311)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: Data was quantified in Fig. 1D.
Results
Specimen 1A: embryonic day 14.5; Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/Gt(ROSA)26Sor+, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. GFP reporter protein was expressed by all enteric neural crest-derived cells.
Structure Level Pattern Image Note
TS22: duodenum Present Regionally restricted 1A merge , 1A S100B Expressed in the myenteric plexus of the duodenum (13+/-4% of total GFP+ cells). All S100b+ cells were also Sox10+.
TS22: enteric nervous system Present Regionally restricted 1A merge , 1A S100B Expressed in the myenteric plexus of the duodenum (13+/-4% of total GFP+ cells). All S100b+ cells were also Sox10+.
Specimen 1B: embryonic day 16.5; Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/Gt(ROSA)26Sor+, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. GFP reporter protein was expressed by all enteric neural crest-derived cells.
Structure Level Pattern Image Note
TS24: duodenum Present 1B S100B , 1B merge Increased expression in the myenteric plexus of the duodenum (>30% of total GFP+ cells). A small proportion of S100b+ cells were Sox10- (15+/-3%).
TS24: myenteric nerve plexus Present 1B S100B , 1B merge Increased expression in the myenteric plexus of the duodenum (>30% of total GFP+ cells). A small proportion of S100b+ cells were Sox10- (15+/-3%).
Specimen 1C: postnatal day 0; Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/Gt(ROSA)26Sor+, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. GFP reporter protein was expressed by all enteric neural crest-derived cells.
Structure Level Pattern Image Note
TS27: duodenum Present 1C S100B , 1C merge Increased expression in the myenteric plexus of the duodenum (around 40% of total GFP+ cells). A small proportion of S100b+ cells were Sox10- (6.7+/-3%).
TS27: myenteric nerve plexus Present 1C S100B , 1C merge Increased expression in the myenteric plexus of the duodenum (around 40% of total GFP+ cells). A small proportion of S100b+ cells were Sox10- (6.7+/-3%).
Specimen not shown: embryonic day 13.5; Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/Gt(ROSA)26Sor+, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. GFP reporter protein was expressed by all enteric neural crest-derived cells.
Structure Level Pattern Image Note
TS21: enteric nervous system Absent
TS21: gut Absent
Specimen not shown: embryonic day 14.5; Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/Gt(ROSA)26Sor+, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. GFP reporter protein was expressed by all enteric neural crest-derived cells.
Structure Level Pattern Image Note
TS22: enteric nervous system Trace Regionally restricted Very few positive cells were expressed in the myenteric plexus of the colon.
TS22: large intestine Trace Regionally restricted Very few positive cells were expressed in the myenteric plexus of the colon.
Specimen not shown: embryonic day 16.5; Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/Gt(ROSA)26Sor+, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. GFP reporter protein was expressed by all enteric neural crest-derived cells.
Structure Level Pattern Image Note
TS24: colon Present Increased expression in the myenteric plexus of the colon (around 5% of total GFP+ cells).
TS24: myenteric nerve plexus Present Increased expression in the myenteric plexus of the colon (around 5% of total GFP+ cells).
Specimen not shown: postnatal day 0; Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/Gt(ROSA)26Sor+, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. GFP reporter protein was expressed by all enteric neural crest-derived cells.
Structure Level Pattern Image Note
TS27: colon Present Increased expression in the myenteric plexus of the colon (>20% of total GFP+ cells).
TS27: myenteric nerve plexus Present Increased expression in the myenteric plexus of the colon (>20% of total GFP+ cells).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory