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Gene Expression Data
Assay Details
Assay
Reference: J:284140 Frum T, et al., TEAD4, YAP1 and WWTR1 prevent the premature onset of pluripotency prior to the 16-cell stage. Development. 2019 Sep 6;146(17):dev179861
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6403731
Gene symbol: Sox2
Gene name: SRY (sex determining region Y)-box 2
Antibody: anti-SOX2 (GT15098)
Detection system: Secondary antibody
Results
Specimen 1A non-mutant: embryonic day 3.0 (more )
Note: 16-cell stage. This embryo was the result of a cross between double heterozygotes. It displays a wild-type staining pattern. It may carry no mutant alleles or it may be heterozygous for either or both Nanog and Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 1A SOX2 upper Expression is detected in the inside cells.
Specimen not shown Nanog null: embryonic day 3.0; Nanogtm1Hoch/Nanogtm1Hoch (more )
Note: 16-cell stage. This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted Expression is detected in the inside cells. No differences were observed between the mutant and non-mutant.
Specimen not shown Oct4 null: embryonic day 3.0; Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Note: 16-cell stage. This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Nanog.
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted Expression is detected in the inside cells. No differences were observed between the mutant and non-mutant.
Specimen 1A Nanog; Oct4 null: embryonic day 3.0; Nanogtm1Hoch/Nanogtm1Hoch, Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Note: 16-cell stage.
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 1A SOX2 lower Expression is detected in the inside cells. No differences were observed between the mutant and non-mutant.
Specimen 1B non-mutant: embryonic day 3.25 (more )
Note: 32-cell stage. This embryo was the result of a cross between double heterozygotes. It displays a wild-type staining pattern. It may carry no mutant alleles or it may be heterozygous for either or both Nanog and Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 1B SOX2 upper Expression is detected in the inside cells.
Specimen not shown Nanog null: embryonic day 3.25; Nanogtm1Hoch/Nanogtm1Hoch (more )
Note: 32-cell stage. This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted Expression is detected in the inside cells. No differences were observed between the mutant and non-mutant.
Specimen not shown Oct4 null: embryonic day 3.25; Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Note: 32-cell stage. This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Nanog.
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted Expression is detected in the inside cells. No differences were observed between the mutant and non-mutant.
Specimen 1B Nanog; Oct4 null: embryonic day 3.25; Nanogtm1Hoch/Nanogtm1Hoch, Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Note: 32-cell stage.
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 1B SOX2 lower Expression is detected in the inside cells. No differences were observed between the mutant and non-mutant.
Specimen 1C non-mutant: embryonic day 3.5 (more )
Note: This embryo was the result of a cross between double heterozygotes. It displays a wild-type staining pattern. It may carry no mutant alleles or it may be heterozygous for either or both Nanog and Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS4: inner cell mass Present 1C upper
Specimen not shown Nanog null: embryonic day 3.5; Nanogtm1Hoch/Nanogtm1Hoch (more )
Note: This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS4: inner cell mass Present No differences in expression were observed between the mutant and non-mutant.
Specimen not shown Oct4 null: embryonic day 3.5; Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Note: This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Nanog.
Structure Level Pattern Image Note
TS4: inner cell mass Present No differences in expression were observed between the mutant and non-mutant.
Specimen 1C Nanog; Oct4 null: embryonic day 3.5; Nanogtm1Hoch/Nanogtm1Hoch, Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Structure Level Pattern Image Note
TS4: inner cell mass Weak 1C lower Expression is weak or undetectable in the double mutant.
Specimen 1E +/GFP: embryonic day 3.75; Nanogtm1Hoch/Nanog+ (more )
Note: Double labeled: red - Sox2; green - Nanog (assay MGI:6403729). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1E SOX2 upper , 1E Merge upper
Specimen 1E Nanog null: embryonic day 3.75; Nanogtm1Hoch/Nanogtm1Hoch (more )
Note: Double labeled: red - Sox2; green - Nanog (assay MGI:6403729). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1E Merge lower , 1E SOX2 lower No differences in expression were observed between the mutant and non-mutant.
Specimen 1F non-mutant: embryonic day 3.75 (more )
Note: Double labeled: red - Sox2; green - Nanog (assay MGI:6403730). This embryo was the result of a cross between double heterozygotes; it may carry no mutant alleles or it may be heterozygous for either or both Nanog and Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1F SOX2 upper , 1F Merge upper
Specimen 1F Oct4 null: embryonic day 3.75; Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Note: Double labeled: red - Sox2; green - Nanog (assay MGI:6403730). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Nanog.
Structure Level Pattern Image Note
TS5: inner cell mass Present 1F SOX2 lower , 1F Merge lower No differences in expression were observed between the mutant and non-mutant.
Specimen 1G +/GFP: embryonic day 4.25; Nanogtm1Hoch/Nanog+ (more )
Note: Double labeled: red - Sox2; green - Nanog (assay MGI:6403729). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS6: epiblast Present 1G Merge upper , 1G SOX2 upper
Specimen 1G Nanog null: embryonic day 4.25; Nanogtm1Hoch/Nanogtm1Hoch (more )
Note: Double labeled: red - Sox2; green - Nanog (assay MGI:6403729). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS6: epiblast Absent 1G SOX2 lower , 1G Merge lower Expression is almost undetectable.
Specimen 1H non-mutant: embryonic day 4.25 (more )
Note: Double labeled: red - Sox2; green - Nanog (assay MGI:6403730). This embryo was the result of a cross between double heterozygotes; it may carry no mutant alleles or it may be heterozygous for either or both Nanog and Pou5f1 (Oct4).
Structure Level Pattern Image Note
TS6: epiblast Present 1H Merge upper , 1H SOX2 upper
Specimen 1H Oct4 null: embryonic day 4.25; Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Note: Double labeled: red - Sox2; green - Nanog (assay MGI:6403730). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Nanog.
Structure Level Pattern Image Note
TS6: epiblast Weak 1H SOX2 lower , 1H Merge lower
Specimen 2A non-mutant: embryonic day 2.5 (more )
Note: Littermate; embryo may be heterozygous for Tead4.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 2A upper
Specimen 2A z null: embryonic day 2.5; Tead4tm1.1Bnno/Tead4tm1.1Bnno (more )
Note: zygotic null
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2A center Precocious expression is observed in the mutant.
Specimen 2A mz null: embryonic day 2.5; Tead4tm1.1Bnno/Tead4tm1.1Bnno (more )
Note: maternal-zygotic null
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2A lower Precocious expression is observed in the mutant. Elimination of the maternal transcript did not exacerbate this phenotype.
Specimen 2B +/-; +/-: embryonic day 2.5; Wwtr1tm1.2Eno/Wwtr1+, Yap1tm1.2Eno/Yap1+ (more )
Note: maternal null for both alleles
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2B upper Precocious expression is observed in the mutant. The embryo carries a wild-type paternal allele, but it is not sufficient to rescue the maternal null phenotype.
Specimen 2B +/-; -/-: embryonic day 2.5; Wwtr1tm1.2Eno/Wwtr1+, Yap1tm1.2Eno/Yap1tm1.2Eno (more )
Note: maternal null for both alleles
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2B center Precocious expression is observed in the mutant. The embryo carries a wild-type paternal allele, but it is not sufficient to rescue the maternal null phenotype.
Specimen 2B -/-; -/-: embryonic day 2.5; Wwtr1tm1.2Eno/Wwtr1tm1.2Eno, Yap1tm1.2Eno/Yap1tm1.2Eno (more )
Note: maternal null for both alleles
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2B lower Precocious expression is observed in the mutant.
Specimen 2C non-mutant: embryonic day 2.0 (more )
Note: Littermate; embryo may be heterozygous for Tead4.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent 2C SOX2 upper
Specimen 2C z null: embryonic day 2.0; Tead4tm1.1Bnno/Tead4tm1.1Bnno (more )
Note: zygotic null
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent 2C SOX2 center
Specimen 2C mz null: embryonic day 2.0; Tead4tm1.1Bnno/Tead4tm1.1Bnno (more )
Note: maternal-zygotic null
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent 2C SOX2 lower
Specimen 2D +/-; +/-: embryonic day 2.0; Wwtr1tm1.2Eno/Wwtr1+, Yap1tm1.2Eno/Yap1+ (more )
Note: maternal null for both alleles
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Weak 2D upper Occasional precocious expression is observed in the mutant. The embryo carries a wild-type paternal allele, but it is not sufficient to rescue the maternal null phenotype.
Specimen 2D +/-; -/-: embryonic day 2.0; Wwtr1tm1.2Eno/Wwtr1+, Yap1tm1.2Eno/Yap1tm1.2Eno (more )
Note: maternal null for both alleles
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Weak 2D center Occasional precocious expression is observed in the mutant. The embryo carries a wild-type paternal allele, but it is not sufficient to rescue the maternal null phenotype.
Specimen 2D -/-; -/-: embryonic day 2.0; Wwtr1tm1.2Eno/Wwtr1tm1.2Eno, Yap1tm1.2Eno/Yap1tm1.2Eno (more )
Note: maternal null for both alleles
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Weak 2D lower Occasional precocious expression is observed in the mutant.
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03/25/2025
MGI 6.24 		The Jackson Laboratory